Xu Yue, Balooch Guive, Chiou Michael, Bekerman Elena, Ritchie Robert O, Longaker Michael T
Pediatric Surgical Research Program and Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.
Biochem Biophys Res Commun. 2007 Jul 27;359(2):311-6. doi: 10.1016/j.bbrc.2007.05.098. Epub 2007 May 25.
Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.
软骨是一种无血管组织，愈合潜力有限，体外软骨细胞的增殖能力也很差。最近，脂肪来源的基质细胞（ASC）因其能够分化为软骨、骨和脂肪，在组织工程应用方面显示出巨大潜力。在本研究中，我们利用ASC建立了早期软骨形成的高密度三维（3D）微团模型系统。这些微团的材料特性在早期软骨形成分化过程中显示出动态和静态弹性模量显著增加。这些数据表明，3D微团培养系统代表了一种早期软骨形成的体外模型，具有与软骨细胞分化力学特性相关的动态细胞信号相互作用。
