Gorke Johnathan T, Okrasa Krzysztof, Louwagie Andrew, Kazlauskas Romas J, Srienc Friedrich
Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA.
J Biotechnol. 2007 Nov 1;132(3):306-13. doi: 10.1016/j.jbiotec.2007.04.001. Epub 2007 Apr 24.
Ring-opening polymerization of five lactones catalyzed by Candida antarctica lipase B in ionic liquids yielded poly(hydroxyalkanoates) of moderate molecular weights up to Mn=13,000. In the ionic liquid 1-butyl-3-methylimidazolium bis(trifluoromethane)-sulfonimide and with a low weight ratio of enzyme to lactone (1:100) we obtained polymers from beta-propiolactone, delta-valerolactone, and epsilon-caprolactone with degrees of polymerization as high as 170, 25, and 85, respectively; oligomers from beta-butyrolactone and gamma-butyrolactone with degrees of polymerization of 5; and a copolymer of beta-propiolactone and beta-butyrolactone with a degree of polymerization of 180. Water-immiscible ionic liquids were superior to water-miscible ionic liquids. Reducing the water content of the enzyme improved the degree of polymerization by as much as 50% for beta-propiolactone and epsilon-caprolactone.
南极假丝酵母脂肪酶B在离子液体中催化五种内酯的开环聚合反应，得到了分子量适中（最高可达Mn = 13,000）的聚羟基脂肪酸酯。在离子液体1-丁基-3-甲基咪唑双（三氟甲烷）磺酰亚胺中，且酶与内酯的重量比很低（1:100）时，我们分别从β-丙内酯、δ-戊内酯和ε-己内酯得到了聚合度高达170、25和85的聚合物；从β-丁内酯和γ-丁内酯得到了聚合度为5的低聚物；以及一种聚合度为180的β-丙内酯和β-丁内酯的共聚物。与水混溶的离子液体相比，与水不混溶的离子液体更具优势。降低酶的含水量，对于β-丙内酯和ε-己内酯，聚合度提高了多达50%。
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