Cao Hong, Han Haobo, Li Guangquan, Yang Jiebing, Zhang Lingfei, Yang Yan, Fang Xuedong, Li Quanshun
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China.
Int J Mol Sci. 2012 Sep 25;13(10):12232-41. doi: 10.3390/ijms131012232.
The ring-opening polymerization of δ-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically evaluated. Through the optimization of reaction conditions, poly(δ-valerolactone) was produced in 97% monomer conversion, with a number-average molecular weight of 2225 g/mol, in toluene at 70 °C for 72 h. This paper has produced a new biocatalyst for the synthesis of poly(δ-valerolactone), and also deeper insight has been gained into the mechanism of thermophilic esterase-catalyzed ring-opening polymerization.
由嗜热古菌富铁嗜热栖热菌（Archaeoglobus fulgidus）来源的嗜热酯酶催化的δ-戊内酯开环聚合反应在有机溶剂中成功进行。系统评估了酶浓度、温度、反应时间和反应介质对单体转化率和产物分子量的影响。通过优化反应条件，在70℃的甲苯中反应72小时，以97%的单体转化率制备了数均分子量为2225 g/mol的聚（δ-戊内酯）。本文制备了一种用于合成聚（δ-戊内酯）的新型生物催化剂，并且对嗜热酯酶催化开环聚合反应的机理有了更深入的认识。
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