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'Turboprep' DNA: ultraquick preparation of plasmid DNA from single colonies for DNA sequencing.

作者信息

Woodford K, Usdin K

机构信息

Section on Genomic Structure and Function, National Institute of Diabetes and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

出版信息

Nucleic Acids Res. 1991 Dec 11;19(23):6652. doi: 10.1093/nar/19.23.6652.

DOI:10.1093/nar/19.23.6652
PMID:1754410
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC329251/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/174f/329251/cde80a213a3d/nar00103-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/174f/329251/cde80a213a3d/nar00103-0284-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/174f/329251/cde80a213a3d/nar00103-0284-a.jpg

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Nucleic Acids Res. 1991 Dec 11;19(23):6652. doi: 10.1093/nar/19.23.6652.
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Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.以磁珠作为固相支持物对基因组DNA和质粒DNA进行直接固相测序。
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Plasmid DNA sequencing using highly degenerate oligonucleotides as primers.以高度简并寡核苷酸为引物的质粒DNA测序。
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[Replication of Streptomyces plasmids: the DNA nucleotide sequence of plasmid pSB 24.2].
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本文引用的文献

1
An effective method for eliminating "artifact banding" when sequencing double-stranded DNA templates.
Biotechniques. 1990 Jul;9(1):46-8.