Tang Qingping, Yang Qidong, Hu Zhongyang, Liu Baoqiong, Shuai Jianzhong, Wang Gaiqing, Liu Zunjing, Xia Jian, Shen Xiangmin
Department of Neurology, Xiang Ya Hospital, Central South University, Changsha 410008, Hunan, China.
Behav Brain Res. 2007 Aug 6;181(2):254-61. doi: 10.1016/j.bbr.2007.04.013. Epub 2007 May 4.
Although we demonstrated willed movement (WM) therapy can facilitate the patients actively participating in the physical activities by cognitive and perceptual stimulation in our previous study, the molecular mechanisms of the willed movement on the patients remains unclear. We initially established the model of WM intervention for rats and identified possible effects of willed movement on motor recovery and on expression of Kainate/alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in ischemia core (IC) and ischemia penumbra (IP) regions of rats after cerebral ischemia reperfusion.
Seventy-two adult male Sprague Dawley rats after successful 120-min period of occlusion of the left middle cerebral artery were selected and randomly divided into three groups: middle cerebral artery occlusion (MCAO), WM and environmental modification (EM). Neurological and neurobehavioral assessments were performed and the rats were killed at various recirculation times after MCAO. Reverse transcription-PCR were used to detect mRNA of GluR1-GluR4 subunit of AMPA receptors in the areas of IC and IP in all adult rats.
Rats following WM intervention showed significantly better acquisition of climbing (every time point tested), forelimb mobility and neurological functions at subacute stage of MCAO. No difference was found in the expression levels of GluR1-GluR4 mRNA among three groups in IC region. However, GluR1 and GluR4 mRNA of rats in group WM were significantly upregulated as compared with rats in group MCAO and group EM in IP region at subacute stage.
Early willed movement treatment can increase the expression level of AMPA receptor subunits and thus might increase synaptic transmission and enhance brain plasticity after focal brain ischemia at the subacute stage.
尽管我们在先前的研究中证明，意志性运动（WM）疗法可通过认知和感知刺激促进患者积极参与体育活动，但意志性运动对患者的分子机制仍不清楚。我们最初建立了大鼠WM干预模型，并确定了意志性运动对大鼠脑缺血再灌注后缺血核心（IC）和缺血半暗带（IP）区域运动恢复以及对海人藻酸/α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体表达的可能影响。
选择72只成年雄性Sprague Dawley大鼠，在成功阻断左大脑中动脉120分钟后，随机分为三组：大脑中动脉闭塞（MCAO）组、WM组和环境改变（EM）组。进行神经和神经行为评估，并在MCAO后的不同再灌注时间处死大鼠。采用逆转录聚合酶链反应（RT-PCR）检测所有成年大鼠IC和IP区域AMPA受体GluR1-GluR4亚基的mRNA。
在MCAO亚急性期，接受WM干预的大鼠在攀爬能力（每个测试时间点）、前肢活动能力和神经功能方面的获得明显更好。IC区域三组之间GluR1-GluR4 mRNA的表达水平没有差异。然而，在亚急性期，WM组大鼠IP区域的GluR1和GluR4 mRNA与MCAO组和EM组大鼠相比显著上调。
早期意志性运动治疗可提高AMPA受体亚基的表达水平，从而可能增加突触传递并增强局灶性脑缺血亚急性期后的脑可塑性。
