Gioia Jason, Highlander Sarah K
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
Vet Microbiol. 2007 Oct 6;124(3-4):298-309. doi: 10.1016/j.vetmic.2007.04.035. Epub 2007 Apr 29.
The ferric uptake regulator (Fur) is an iron-dependent transcriptional regulator that regulates genes related to iron acquisition, oxidative stress response, and various other functions. Transcription of fur is typically self-regulating and sensitive to iron and oxidative stress. Following the identification of a fur gene in the genome of the bovine pathogen Mannheimia haemolytica, an attempt was made to characterize the transcriptional control of M. haemolytica fur. Northern blotting, RT-PCR, and primer extension were done to determine that M. haemolytica fur is transcribed using three distinct promoters, two of which are located within the upstream fldA gene. The third promoter is located upstream of a conserved hypothetical protein and drives transcription of a tricistronic message. Quantitative real time PCR experiments indicated that unlike current models of Fur regulation, M. haemolytica fur transcription is unchanged by iron depletion at logarithmic phase and repressed by iron depletion at stationary phase.
铁摄取调节蛋白（Fur）是一种铁依赖性转录调节因子，可调节与铁获取、氧化应激反应及各种其他功能相关的基因。fur的转录通常是自我调节的，且对铁和氧化应激敏感。在牛病原体溶血曼氏杆菌的基因组中鉴定出fur基因后，人们尝试对溶血曼氏杆菌fur的转录调控进行表征。进行了Northern印迹、逆转录-聚合酶链反应（RT-PCR）和引物延伸实验，以确定溶血曼氏杆菌fur使用三个不同的启动子进行转录，其中两个位于上游fldA基因内。第三个启动子位于一个保守的假定蛋白上游，并驱动一个多顺反子信息的转录。定量实时PCR实验表明，与当前的Fur调节模型不同，溶血曼氏杆菌fur转录在对数期不受铁耗竭影响，而在稳定期受铁耗竭抑制。
