Flynn Lauren, Prestwich Glenn D, Semple John L, Woodhouse Kimberly A
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ont., Canada.
Biomaterials. 2007 Sep;28(26):3834-42. doi: 10.1016/j.biomaterials.2007.05.002. Epub 2007 May 16.
A tissue-engineered adipose substitute would have numerous applications in plastic and reconstructive surgery. This work involves the characterization of the in vitro cellular response of primary human adipose-derived stem cells (ASC) to three dimensional, naturally derived scaffolds. To establish a more thorough understanding of the influence of the scaffold environment on ASC, we have designed several different soft tissue scaffolds composed of decellularized human placenta and crosslinked hyaluronan (XLHA). The cellular organization within the scaffolds was characterized using confocal microscopy. Adipogenic differentiation was induced and the ASC response was characterized in terms of glycerol-3-phosphate dehydrogenase (GPDH) activity and intracellular lipid accumulation. The results indicate that the scaffold environment impacts the ASC response and that the adipogenic differentiation of the ASC was augmented in the non-adhesive XLHA gels.
一种组织工程化脂肪替代物在整形和重建手术中将有众多应用。这项工作涉及对原代人脂肪来源干细胞（ASC）对三维天然衍生支架的体外细胞反应进行表征。为了更全面地了解支架环境对ASC的影响，我们设计了几种由脱细胞人胎盘和交联透明质酸（XLHA）组成的不同软组织支架。使用共聚焦显微镜对支架内的细胞组织进行表征。诱导脂肪生成分化，并根据甘油-3-磷酸脱氢酶（GPDH）活性和细胞内脂质积累来表征ASC反应。结果表明，支架环境影响ASC反应，并且在非粘性XLHA凝胶中ASC的脂肪生成分化增强。
