Phan Tung Gia, Okitsu Shoko, Maneekarn Niwat, Ushijima Hiroshi
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Infect Genet Evol. 2007 Sep;7(5):656-63. doi: 10.1016/j.meegid.2007.05.001. Epub 2007 May 6.
G9 rotavirus is recognized as the emerging genotype spreading around the world. The rapidly increasing detection of this virus in association with the genetic heterogeneity raises questions regarding its origin and epidemiological importance. A total of 380 sequences of rotavirus G9 strains including our sequence data from Vietnam and Japan, which were detected from 1983 to 2006 in five different continents, were collected from GenBank to investigate their heterogeneity and evolution. A novel nomenclature for G9 rotaviruses is proposed, in which these viruses are clustered into 6 lineages with 11 sublineages. Multiple amino acid substitutions of VP7 specific for lineages and sublineages were found. Interestingly, six short amino acid motifs correctly defined phylogenetic lineages and sublineages. Another interesting finding was the identification of recombinant G9 rotavirus, bearing different genotype sequence. In view of rotavirus evolution, this report is an additional evidence to support the notion that there might exist a genomic relatedness between human and porcine rotaviruses.
G9轮状病毒被认为是在全球传播的新兴基因型。该病毒检测率的迅速上升及其基因异质性引发了关于其起源和流行病学重要性的问题。从GenBank收集了总共380个G9轮状病毒株序列，包括我们来自越南和日本的序列数据，这些序列是1983年至2006年在五个不同大陆检测到的，以研究它们的异质性和进化。提出了一种G9轮状病毒的新命名法，其中这些病毒被聚类为6个谱系和11个亚谱系。发现了特定于谱系和亚谱系的VP7的多个氨基酸替换。有趣的是，六个短氨基酸基序正确地定义了系统发育谱系和亚谱系。另一个有趣的发现是鉴定出携带不同基因型序列的重组G9轮状病毒。鉴于轮状病毒的进化，本报告为支持人和猪轮状病毒之间可能存在基因组相关性这一观点提供了额外证据。
