[趋化因子CCL20和趋化因子受体CXCR4在尖锐湿疣皮损中的表达]
[Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions].
作者信息
Feng Jin-yun, Peng Zhen-hui, Tang Xiao-ping, Wang Mei-ju, Liu Yu-ping
机构信息
Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Apr;27(4):418-20.
OBJECTIVE
To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis.
METHODS
Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting.
RESULTS
Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis.
CONCLUSION
The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.
目的
检测尖锐湿疣（CA）感染表皮和正常表皮中CCL20和CXCR4的表达，并研究其表达对CA表皮中朗格汉斯细胞的影响。
方法
使用Affymetrix寡核苷酸微阵列HG-U 133A 2.0检测3个表皮CA病变组织和3个正常表皮标本中CCL20和CXCR4的基因表达，并通过蛋白质印迹法检测这些标本中CCL20和CXCR4的蛋白水平。
结果
微阵列分析显示，与正常标本相比，3个表皮CA病变组织中CCL20和CXCR4的mRNA表达明显下调。蛋白质印迹分析表明，CA病变组织中CCL20和CXCR4的蛋白表达明显低于正常表皮。
结论
表皮CA病变组织中CCL20和CXCR4的蛋白和mRNA表达明显下调，这可能导致这些病变组织中朗格汉斯细胞数量减少和回流紊乱。
Zotero 插件