Lucier Jean-François, Perreault Jonathan, Noël Jean-François, Boire Gilles, Perreault Jean-Pierre
RNA Group/Groupe ARN, Département de microbiologie et infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada.
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W269-74. doi: 10.1093/nar/gkm313. Epub 2007 Jun 1.
Mobile genetic elements have significantly contributed to the shaping of mammalian genomes. The RTAnalyzer software tracks sequences of retrotransposed origin by scoring the signature results from an L1-mediated insertion within a genome. More specifically, a sequence of interest is searched for in genomic databases using BLAST. Each hit, along with additional 5' and 3' sequences of pre-defined lengths, is saved. RTAnalyzer searches for specific L1 retrotransposition signatures (i.e. target site duplication, endonuclease cleavage site and poly(A)), and then calculates an overall retrotransposition score. This score represents the likelihood of a given sequence originating from a retrotransposition event involving the L1 machinery. RTAnalyzer may be used under GNU public license, and is available at http://www.riboclub.org/rtanalyzer.
移动遗传元件对哺乳动物基因组的形成起到了重要作用。RTAnalyzer软件通过对基因组中L1介导插入的特征结果进行评分，来追踪反转录转座起源的序列。更具体地说，使用BLAST在基因组数据库中搜索感兴趣的序列。每个匹配结果以及预定义长度的额外5'和3'序列都会被保存下来。RTAnalyzer搜索特定的L1反转录转座特征（即靶位点重复、内切酶切割位点和聚腺苷酸），然后计算总体反转录转座分数。该分数代表给定序列源自涉及L1机制的反转录转座事件的可能性。RTAnalyzer可在GNU公共许可证下使用，可从http://www.riboclub.org/rtanalyzer获取。
