Rabon H W, Roland D A, Clark A J
Department of Poultry Science, Auburn University, Alabama 36849.
Poult Sci. 1991 Nov;70(11):2280-3. doi: 10.3382/ps.0702280.
Uterine calcium-binding protein (CaBP) activity was compared at oviposition and 14 h postoviposition in nonlaying (NL) hens and hens laying a high incidence of shell-less (SL) or hard-shelled (HS) eggs fed a commercial laying diet. Epithelial cells were removed from uteri of NL, SL, and HS hens and homogenized in Tris buffer, pH 7.4. Proteins in homogenate were fractionated by the addition of ammonium sulfate and centrifugation, separated by Sephadex chromatography, and measured by ultraviolet spectrophotometry at A280. Calcium-binding protein activity was determined using a 45Ca-chelex binding assay. Two peaks of CaBP activity were detected and designated CaBP-a (28,000 Da) and CaBP-b (16,000 Da). Uterine CaBP-b may differ from CaBP-a only in Ca content. The activities of CaBP (a and b) were higher (P less than or equal to .05) in HS and SL hens than in NL hens at 0 h. There was no significant difference (0 h) between CaBP activity (a versus b) of either HS or SL hens. There was also no difference (0 h) of CaBP-a or CaBP-b activity when SL and HS hens were compared. Uterine CaBP-a activity was lower (P less than or equal to .05) in SL hens than HS hens at 14 h. Under normal dietary conditions, the level of CaBP activity at 14 h may be associated with SL egg production.
在产蛋和产蛋后14小时，对非产蛋（NL）母鸡以及产软壳（SL）或硬壳（HS）蛋比例高的母鸡（喂食商业产蛋日粮）的子宫钙结合蛋白（CaBP）活性进行了比较。从NL、SL和HS母鸡的子宫中取出上皮细胞，在pH 7.4的Tris缓冲液中匀浆。通过添加硫酸铵和离心对匀浆中的蛋白质进行分级分离，通过葡聚糖凝胶色谱法进行分离，并在280nm处通过紫外分光光度法进行测定。使用45Ca-螯合树脂结合试验测定钙结合蛋白活性。检测到两个CaBP活性峰，分别命名为CaBP-a（28,000 Da）和CaBP-b（16,000 Da）。子宫CaBP-b可能仅在钙含量上与CaBP-a不同。在0小时时，HS和SL母鸡中CaBP（a和b）的活性高于NL母鸡（P≤0.05）。HS或SL母鸡的CaBP活性（a与b）在0小时时没有显著差异。比较SL和HS母鸡时，CaBP-a或CaBP-b活性在0小时时也没有差异。在14小时时，SL母鸡的子宫CaBP-a活性低于HS母鸡（P≤0.05）。在正常饮食条件下，14小时时CaBP活性水平可能与软壳蛋的产生有关。
