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Notch激活刺激Su(H)/CSL与靶增强子的瞬时和选择性结合。

Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers.

作者信息

Krejcí Alena, Bray Sarah

机构信息

Department of Physiology Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom.

出版信息

Genes Dev. 2007 Jun 1;21(11):1322-7. doi: 10.1101/gad.424607.

DOI:10.1101/gad.424607
PMID:17545467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1877745/
Abstract

The CSL [CBF1/Su(H)/Lag2] proteins [Su(H) in Drosophila] are implicated in repression and activation of Notch target loci. Prevailing models imply a static association of these DNA-binding transcription factors with their target enhancers. Our analysis of Su(H) binding and chromatin-associated features at 11 E(spl) Notch target genes before and after Notch revealed large differences in Su(H) occupancy at target loci that correlated with the presence of polymerase II and other marks of transcriptional activity. Unexpectedly, Su(H) occupancy was significantly and transiently increased following Notch activation, suggesting a more dynamic interaction with targets than hitherto proposed.

摘要

CSL[CBF1/Su(H)/Lag2]蛋白（果蝇中的Su(H)）与Notch靶基因座的抑制和激活有关。普遍的模型表明这些DNA结合转录因子与其靶增强子存在静态关联。我们对11个E(spl)Notch靶基因在Notch激活前后的Su(H)结合及染色质相关特征进行分析，发现靶基因座上Su(H)的占据情况存在很大差异，这与聚合酶II的存在及其他转录活性标记相关。出乎意料的是，Notch激活后Su(H)的占据显著且短暂增加，这表明与靶标的相互作用比迄今所提出的更为动态。

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Genes Dev. 2007 Jun 1;21(11):1322-7. doi: 10.1101/gad.424607.
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本文引用的文献

1
Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription.高阶Notch复合体的协同组装作为一种开关来诱导转录。
Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2103-8. doi: 10.1073/pnas.0611092104. Epub 2007 Feb 6.
2
Notch signalling: a simple pathway becomes complex.Notch信号通路：一条简单的通路变得复杂起来。
Nat Rev Mol Cell Biol. 2006 Sep;7(9):678-89. doi: 10.1038/nrm2009.
3
Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA.与DNA结合的CSL-Notch-Mastermind三元复合物的晶体结构。
Cell. 2006 Mar 10;124(5):985-96. doi: 10.1016/j.cell.2006.01.035.
4
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.MAML共激活因子募集至Notch转录复合物过程中协同作用的结构基础。
Cell. 2006 Mar 10;124(5):973-83. doi: 10.1016/j.cell.2005.12.037.
5
A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappaB-dependent gene activity.启动子结合二聚体与核质二聚体之间的高动态平衡控制着NF-κB依赖性基因活性。
EMBO J. 2006 Feb 22;25(4):798-810. doi: 10.1038/sj.emboj.7600977. Epub 2006 Feb 9.
6
Transcription in four dimensions: nuclear receptor-directed initiation of gene expression.四维转录：核受体介导的基因表达起始
EMBO Rep. 2006 Feb;7(2):161-7. doi: 10.1038/sj.embor.7400626.
7
Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors.无毛蛋白介导的Notch靶基因抑制需要Gro和CtBP共抑制因子的联合活性。
Mol Cell Biol. 2005 Dec;25(23):10433-41. doi: 10.1128/MCB.25.23.10433-10441.2005.
8
RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.RBP-Jκ/SHARP招募CtIP/CtBP共抑制因子以沉默Notch靶基因。
Mol Cell Biol. 2005 Dec;25(23):10379-90. doi: 10.1128/MCB.25.23.10379-10390.2005.
9
Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin.组蛋白变体H2A.Z标记常染色质中活跃基因和非活跃基因的5'端。
Cell. 2005 Oct 21;123(2):233-48. doi: 10.1016/j.cell.2005.10.002.
10
Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone loss.Htz1的全基因组动态变化，Htz1是一种组蛋白H2A变体，通过组蛋白缺失使抑制/基础启动子为激活做好准备。
Cell. 2005 Oct 21;123(2):219-31. doi: 10.1016/j.cell.2005.08.036.