Notch激活刺激Su(H)/CSL与靶增强子的瞬时和选择性结合。
Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers.
作者信息
Krejcí Alena, Bray Sarah
机构信息
Department of Physiology Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, United Kingdom.
出版信息
Genes Dev. 2007 Jun 1;21(11):1322-7. doi: 10.1101/gad.424607.
Abstract
The CSL [CBF1/Su(H)/Lag2] proteins [Su(H) in Drosophila] are implicated in repression and activation of Notch target loci. Prevailing models imply a static association of these DNA-binding transcription factors with their target enhancers. Our analysis of Su(H) binding and chromatin-associated features at 11 E(spl) Notch target genes before and after Notch revealed large differences in Su(H) occupancy at target loci that correlated with the presence of polymerase II and other marks of transcriptional activity. Unexpectedly, Su(H) occupancy was significantly and transiently increased following Notch activation, suggesting a more dynamic interaction with targets than hitherto proposed.
摘要
CSL[CBF1/Su(H)/Lag2]蛋白（果蝇中的Su(H)）与Notch靶基因座的抑制和激活有关。普遍的模型表明这些DNA结合转录因子与其靶增强子存在静态关联。我们对11个E(spl)Notch靶基因在Notch激活前后的Su(H)结合及染色质相关特征进行分析，发现靶基因座上Su(H)的占据情况存在很大差异，这与聚合酶II的存在及其他转录活性标记相关。出乎意料的是，Notch激活后Su(H)的占据显著且短暂增加，这表明与靶标的相互作用比迄今所提出的更为动态。
相似文献
1
Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers.Notch激活刺激Su(H)/CSL与靶增强子的瞬时和选择性结合。
Genes Dev. 2007 Jun 1;21(11):1322-7. doi: 10.1101/gad.424607.
2
Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor.具有协同作用的 Notch 结合位点的增强子对 Hairless 共抑制因子的调控更具抗性。
PLoS Genet. 2021 Sep 24;17(9):e1009039. doi: 10.1371/journal.pgen.1009039. eCollection 2021 Sep.
3
Structural and functional analysis of the repressor complex in the Notch signaling pathway of Drosophila melanogaster.果蝇 Notch 信号通路中阻遏复合物的结构与功能分析。
Mol Biol Cell. 2011 Sep;22(17):3242-52. doi: 10.1091/mbc.E11-05-0420. Epub 2011 Jul 7.
4
Molecular analysis of the notch repressor-complex in Drosophila: characterization of potential hairless binding sites on suppressor of hairless.果蝇 Notch 抑制复合物的分子分析：在抑制毛状基因的蛋白上鉴定潜在的 hairless 结合位点。
PLoS One. 2011;6(11):e27986. doi: 10.1371/journal.pone.0027986. Epub 2011 Nov 18.
5
Chromatin signatures at Notch-regulated enhancers reveal large-scale changes in H3K56ac upon activation.Notch调节增强子处的染色质特征揭示了激活后H3K56ac的大规模变化。
EMBO J. 2015 Jul 14;34(14):1889-904. doi: 10.15252/embj.201489923. Epub 2015 Jun 11.
6
Three distinct mechanisms, Notch instructive, permissive, and independent, regulate the expression of two different pericardial genes to specify cardiac cell subtypes.三种不同的机制，即 Notch 指令性、许可性和独立性，调节两种不同的心包膜基因的表达，以指定心脏细胞亚型。
PLoS One. 2020 Oct 27;15(10):e0241191. doi: 10.1371/journal.pone.0241191. eCollection 2020.
7
Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.果蝇 Hairless/Su(H) 异二聚体的核质穿梭作为调节 Notch 依赖性转录的一种手段。
Biochim Biophys Acta Mol Cell Res. 2019 Oct;1866(10):1520-1532. doi: 10.1016/j.bbamcr.2019.07.008. Epub 2019 Jul 19.
8
Regulation of expression of Vg and establishment of the dorsoventral compartment boundary in the wing imaginal disc by Suppressor of Hairless.无毛抑制因子对翅成虫盘Vg表达的调控及背腹区室边界的建立
Dev Biol. 2006 Jan 1;289(1):77-90. doi: 10.1016/j.ydbio.2005.10.008. Epub 2005 Nov 22.
9
Complex genetic interactions of novel Suppressor of Hairless alleles deficient in co-repressor binding.新型 Hairless 抑制基因等位基因的复杂遗传相互作用，这些等位基因缺乏与共抑制因子的结合。
PLoS One. 2018 Mar 6;13(3):e0193956. doi: 10.1371/journal.pone.0193956. eCollection 2018.
10
Specificity of Notch pathway activation: twist controls the transcriptional output in adult muscle progenitors.Notch 通路激活的特异性： twist 控制成体肌肉祖细胞中的转录输出。
Development. 2010 Aug;137(16):2633-42. doi: 10.1242/dev.053181. Epub 2010 Jul 7.
引用本文的文献
1
The Drosophila histone variant H2Av facilitates Notch signaling activity in a two-tier regulatory fashion.果蝇组蛋白变体H2Av以两层调节方式促进Notch信号活性。
Cell Commun Signal. 2025 Jul 1;23(1):322. doi: 10.1186/s12964-025-02333-6.
2
Su(H) Modulates Enhancer Transcriptional Bursting in Prelude to Gastrulation.Su(H) 调节原肠胚形成前期增强子转录爆发。
Cells. 2024 Oct 24;13(21):1759. doi: 10.3390/cells13211759.
3
Notch induces transcription by stimulating release of paused RNA polymerase II.Notch 通过刺激暂停的 RNA 聚合酶 II 的释放来诱导转录。
Genes Dev. 2024 Nov 27;38(21-24):965-978. doi: 10.1101/gad.352108.124.
4
Advances in the role of membrane-bound transcription factors in carcinogenesis and therapy.膜结合转录因子在致癌作用和治疗中的作用进展。
Discov Oncol. 2024 Oct 15;15(1):559. doi: 10.1007/s12672-024-01414-1.
5
The cellular Notch1 protein promotes KSHV reactivation in an Rta-dependent manner.细胞 Notch1 蛋白以 Rta 依赖的方式促进 KSHV 的重新激活。
J Virol. 2024 Aug 20;98(8):e0078824. doi: 10.1128/jvi.00788-24. Epub 2024 Jul 8.
6
Notch signaling regulates UNC5B to suppress endothelial proliferation, migration, junction activity, and retinal plexus branching.Notch 信号通路调控 UNC5B 抑制血管内皮细胞的增殖、迁移、连接活动和视网膜丛的分支。
Sci Rep. 2024 Jun 13;14(1):13603. doi: 10.1038/s41598-024-64375-z.
7
Novel Genome-Engineered Alleles Differentially Affect Lateral Inhibition and Cell Dichotomy Processes during Bristle Organ Development.新型基因组工程改造的等位基因在刚毛器官发育过程中对侧抑制和细胞二分过程有差异影响。
Genes (Basel). 2024 Apr 26;15(5):552. doi: 10.3390/genes15050552.
8
Dynamic modes of Notch transcription hubs conferring memory and stochastic activation revealed by live imaging the co-activator Mastermind.活细胞成像揭示共激活因子 Mastermind 赋予 Notch 转录枢纽记忆和随机激活的动态模式。
Elife. 2024 May 10;12:RP92083. doi: 10.7554/eLife.92083.
9
Comprehensive genomic features indicative for Notch responsiveness.具有 Notch 反应性的综合基因组特征。
Nucleic Acids Res. 2024 May 22;52(9):5179-5194. doi: 10.1093/nar/gkae292.
10
Changes in searching behaviour of CSL transcription complexes in Notch active conditions.CSL 转录复合物在 Notch 激活条件下的搜索行为变化。
Life Sci Alliance. 2023 Dec 14;7(3). doi: 10.26508/lsa.202302336. Print 2024 Mar.
本文引用的文献
1
Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription.高阶Notch复合体的协同组装作为一种开关来诱导转录。
Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2103-8. doi: 10.1073/pnas.0611092104. Epub 2007 Feb 6.
2
Notch signalling: a simple pathway becomes complex.Notch信号通路：一条简单的通路变得复杂起来。
Nat Rev Mol Cell Biol. 2006 Sep;7(9):678-89. doi: 10.1038/nrm2009.
3
Crystal structure of the CSL-Notch-Mastermind ternary complex bound to DNA.与DNA结合的CSL-Notch-Mastermind三元复合物的晶体结构。
Cell. 2006 Mar 10;124(5):985-96. doi: 10.1016/j.cell.2006.01.035.
4
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.MAML共激活因子募集至Notch转录复合物过程中协同作用的结构基础。
Cell. 2006 Mar 10;124(5):973-83. doi: 10.1016/j.cell.2005.12.037.
5
A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-kappaB-dependent gene activity.启动子结合二聚体与核质二聚体之间的高动态平衡控制着NF-κB依赖性基因活性。
EMBO J. 2006 Feb 22;25(4):798-810. doi: 10.1038/sj.emboj.7600977. Epub 2006 Feb 9.
6
Transcription in four dimensions: nuclear receptor-directed initiation of gene expression.四维转录：核受体介导的基因表达起始
EMBO Rep. 2006 Feb;7(2):161-7. doi: 10.1038/sj.embor.7400626.
7
Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors.无毛蛋白介导的Notch靶基因抑制需要Gro和CtBP共抑制因子的联合活性。
Mol Cell Biol. 2005 Dec;25(23):10433-41. doi: 10.1128/MCB.25.23.10433-10441.2005.
8
RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.RBP-Jκ/SHARP招募CtIP/CtBP共抑制因子以沉默Notch靶基因。
Mol Cell Biol. 2005 Dec;25(23):10379-90. doi: 10.1128/MCB.25.23.10379-10390.2005.
9
Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin.组蛋白变体H2A.Z标记常染色质中活跃基因和非活跃基因的5'端。
Cell. 2005 Oct 21;123(2):233-48. doi: 10.1016/j.cell.2005.10.002.
10
Genome-wide dynamics of Htz1, a histone H2A variant that poises repressed/basal promoters for activation through histone loss.Htz1的全基因组动态变化，Htz1是一种组蛋白H2A变体，通过组蛋白缺失使抑制/基础启动子为激活做好准备。
Cell. 2005 Oct 21;123(2):219-31. doi: 10.1016/j.cell.2005.08.036.
Zotero 插件