Xu Xia, Keefer Larry K, Ziegler Regina G, Veenstra Timothy D
SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702, USA.
Nat Protoc. 2007;2(6):1350-5. doi: 10.1038/nprot.2007.176.
The ability to measure estrogen metabolites (EMs) quantitatively is important for investigating their individual roles in cancer screening, treatment and prevention, as well as in a host of other hormone-related disorders. In this protocol we describe a method that is capable of quantitating 15 distinct EMs in urine. Endogenous EMs are quantitatively measured using a liquid chromatography-tandem mass spectrometry method in which the spectrometer operates in a selected reaction monitoring mode. This method is capable of quantifying estrone and its 2-, and 4- and 16alpha-hydroxy and its 2-, 4-methoxy derivatives, and 2-hydroxyestrone-3-methyl ether; 17beta-estradiol and its 2-hydroxy, and 2- and 4-methoxy derivates, and estriol, 16-epiestriol, 17-epiestriol, and 16-ketoestradiol. The method requires only 0.5 ml of urine and approximately 60 urine samples can be quantitatively analyzed per week.
定量测量雌激素代谢物（EMs）的能力对于研究它们在癌症筛查、治疗和预防以及许多其他激素相关疾病中的个体作用至关重要。在本方案中，我们描述了一种能够定量尿液中15种不同EMs的方法。使用液相色谱-串联质谱法对内源性EMs进行定量测量，其中光谱仪在选择反应监测模式下运行。该方法能够定量雌酮及其2-、4-和16α-羟基以及其2-、4-甲氧基衍生物，以及2-羟基雌酮-3-甲醚；17β-雌二醇及其2-羟基、2-和4-甲氧基衍生物，以及雌三醇、16-表雌三醇、17-表雌三醇和16-酮雌二醇。该方法仅需0.5毫升尿液，每周大约可定量分析60份尿液样本。
