Wendt Luiz Roberto, Osvaldt Alessandro Bersch, Bersch Vivian Pierre, Schumacher Rita de Cássia, Edelweiss Maria Isabel Albano, Rohde Luiz
Surgery Post-Graduation Program, UFRGS, Brazil.
Acta Cir Bras. 2007 May-Jun;22(3):202-9. doi: 10.1590/s0102-86502007000300008.
To evaluate the effects of alcohol and caffeine in a pancreatic carcinogenesis mouse model induced by 7,12-dimethylbenzantracene (DMBA), according to the PanIN classification system.
120 male, Mus musculus, CF-1 mice were divided into four groups. Animals received either water or caffeine or alcohol or alcohol + caffeine in their drinking water. In all animals, 1 mg of DMBA was implanted into the head of the pancreas. After 30 days, euthanasia was performed; excised pancreata were then fixed in formalin, stained with hematoxylin-eosin and categorized as follows: normal ducts, reactive hyperplasia, PanIN-1A, PanIN-1B, PanIN-2, PanIN-3 or adenocarcinoma.
PanIN lesions were verified in all groups. Adenocarcinoma was detected in 15% of animals in the caffeine group, 16.6% in the water group, 23.8% in the alcohol + caffeine group and 52.9% in the alcohol group (P<0.05).
The experimental pancreatic carcinogenesis mouse model using DMBA effectively induces PanIN lesions and pancreatic adenocarcinoma. This study verified the association between alcohol use and pancreatic adenocarcinoma; caffeine did not present the same effect.
根据胰腺上皮内瘤变（PanIN）分类系统，评估酒精和咖啡因在7,12 - 二甲基苯并蒽（DMBA）诱导的胰腺癌发生小鼠模型中的作用。
将120只雄性CF - 1小鼠分为四组。动物在饮用水中分别给予水、咖啡因、酒精或酒精 + 咖啡因。所有动物均在胰腺头部植入1 mg DMBA。30天后实施安乐死；然后将切除的胰腺固定在福尔马林中，用苏木精 - 伊红染色，并分类如下：正常导管、反应性增生、PanIN - 1A、PanIN - 1B、PanIN - 2、PanIN - 3或腺癌。
所有组均证实存在PanIN病变。咖啡因组15%的动物检测到腺癌，水组为16.6%，酒精 + 咖啡因组为23.8%，酒精组为52.9%（P<0.05）。
使用DMBA的实验性胰腺癌发生小鼠模型可有效诱导PanIN病变和胰腺腺癌。本研究证实了饮酒与胰腺腺癌之间的关联；咖啡因未表现出相同的作用。
