[全唾液中白细胞介素-1β和基质金属蛋白酶-8在牙周炎患者中的检测及意义]

[Detection and significance of IL-1beta and MMP-8 in patients with periodontitis of whole unstimulated saliva].

作者信息

Wu Ying, Shu Rong, Shen Min-hua, Ge Lin-hua

机构信息

Department of Periodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.

出版信息

Shanghai Kou Qiang Yi Xue. 2007 Apr;16(2):127-30.

PMID:17546376

Abstract

PURPOSE

To detect the role of IL-1beta and MMP-8 by ELISA in whole unstimulated saliva of patients with different type of periodontitis and to determine whether they are possible specific biomarkers of periodontitis.

METHODS

A total of 80 saliva samples were collected from 28 GCP patients, 28 GAgP patients and 24 healthy subjects. The levels of IL-1beta and MMP-8 were respectively measured by ELISA kits. The data were analysed statistically with one-way ANOVA.

RESULTS

The mean salivary levels of IL-1beta in GCP group,GAgP group and control group were 144.40 pg/ml+/-150.70 pg/ml+/-72.56 pg/ml+/-69.36 pg/ml and 65.96 pg/ml+/-71.18 pg/ml respectively, There was no significance difference between the three groups (P>0.05); GCP and GAgP groups presented significantly elevated salivary levels of MMP-8 than that of the control group, which was (576.89+/-559.24) ng/ml, (420.93+/-533.73)ng/ml and (151.49+/-216.38) ng/ml respectively (P<0.05), while there was no significant difference between GCP and GAgP group (P>0.05).

CONCLUSION

Salivary levels of MMP-8 may be a biomarker for diagnosis for periodontitis while IL-1beta needs to be further testified.

摘要

目的

通过酶联免疫吸附测定（ELISA）检测不同类型牙周炎患者全唾液中白细胞介素-1β（IL-1β）和基质金属蛋白酶-8（MMP-8）的作用，并确定它们是否可能是牙周炎的特异性生物标志物。

方法

从28例慢性牙周炎（GCP）患者、28例侵袭性牙周炎（GAgP）患者和24例健康受试者中收集了80份唾液样本。分别使用ELISA试剂盒测量IL-1β和MMP-8的水平。数据采用单因素方差分析进行统计学分析。

结果

GCP组、GAgP组和对照组唾液中IL-1β的平均水平分别为144.40 pg/ml±150.70 pg/ml、72.56 pg/ml±69.36 pg/ml和65.96 pg/ml±71.18 pg/ml，三组之间无显著差异（P>0.05）；GCP组和GAgP组唾液中MMP-8水平显著高于对照组，分别为(576.89±559.24) ng/ml、(420.93±533.73)ng/ml和(151.49±216.38) ng/ml（P<0.05），而GCP组和GAgP组之间无显著差异（P>0.05）。