Kocamemi B Alpaslan, Ceçen F
Department of Environmental Engineering, University of Marmara, Kuyubasi, Istanbul, Turkey.
Water Sci Technol. 2007;55(8-9):67-73. doi: 10.2166/wst.2007.243.
The aim was to investigate the inhibitory effect of the xenobiotic 1,2-DCA on nitrification during the cometabolic degradation in a packed bed nitrifying biofilm reactor. This xenobiotic inhibited primarily the conversion of NH4-N to hydroxylamine by binding to the AMO enzyme. It had no inhibitory effect on the conversion of nitrite to nitrate. At high NH4-N loadings, the presence of 1,2-DCA inhibited NH4-N utilisation more severely than at low loadings. The suppressing effect of 1,2-DCA on NH4-N utilisation was found to be reversible due to the ability of cells to recover from inhibition. These results could fill a gap in the literature about the potential use of nitrifying biofilm systems for cometabolic treatment of 1,2-DCA and could be useful in the design of engineered 1,2-DCA remediation/treatment in biofilm reactors.
目的是研究在填充床硝化生物膜反应器中，异生物质1,2-二氯乙烷在共代谢降解过程中对硝化作用的抑制效果。这种异生物质主要通过与氨单加氧酶（AMO）结合来抑制NH4-N向羟胺的转化。它对亚硝酸盐向硝酸盐的转化没有抑制作用。在高NH4-N负荷下，1,2-二氯乙烷的存在比在低负荷下更严重地抑制了NH4-N的利用。由于细胞具有从抑制中恢复的能力，发现1,2-二氯乙烷对NH4-N利用的抑制作用是可逆的。这些结果可以填补文献中关于硝化生物膜系统对1,2-二氯乙烷共代谢处理潜在用途的空白，并且可能有助于生物膜反应器中工程化1,2-二氯乙烷修复/处理的设计。
