Liu Wei, Zhu Yun-kui, Ma Li
Department of Respiratory, Lanzhou General Hospital, Lanzhou 730050, China.
Zhonghua Nei Ke Za Zhi. 2007 Mar;46(3):213-6.
To explore the level of mononuclear cell oxidative DNA damage and the total antioxidative capacity (TAC) in patients with tuberculous pleurisy. To evaluate the in vitro repair effects of melatonin on mononuclear cell DNA damage in pleural effusion of the patients.
The mononuclear cell DNA damages in pleural effusion and peripheral blood of 28 patients with tuberculous pleurisy and in peripheral blood of 25 healthy persons were detected by single cell gel electrophoresis (comet%). The levels of TAC in supernatant of pleural effusion and blood plasma of 28 patients and in blood plasma of 25 healthy persons were measured by o-phenanthroline colorimetric analysis. The mononuclear cells from pleural effusions of 20 patients were treated by melatonin in vitro.
The comet percentage of mononuclear cells in pleural effusion from patients with tuberculous pleurisy was (41.3 +/- 14.5)%, which was higher than in peripheral blood (21.2 +/- 4.2)%, P < 0.01. The level of TAC in supernatant of pleural effusion was (5.17 +/- 1.19) U/ml, which was lower than in blood plasma (8.66 +/- 1.59) U/ml, P < 0.01. There was a negative correlation between the comet percentage of mononuclear cells and the level of TAC (r = -0.425, P < 0.05) in pleural effusion. The comet percentage of mononuclear cells in peripheral blood from patients was (21.2 +/- 4.2)%, which was higher than from controls (8.9 +/- 3.7)%, P < 0.01. The level of TAC in blood plasma was (8.66 +/- 1.59) U/ml, which was lower than from the controls (10.61 +/- 1.39) U/ml, P < 0.01. After treatment with melatonin, the comet percentage of mononuclear cells in pleural effusion decreased from (40.8 +/- 9.3)% to (11.0 +/- 3.7)%, P < 0.01.
There are oxidative DNA damage and oxidation/antioxidation imbalance in patients with tuberculous pleurisy, particularly in the diseased pleural cavity. Melatonin can facilitate, probably by its antioxidative effects, the in vitro repair of the damaged DNA of mononuclear cells from pleural effusion of patients with tuberculous pleurisy.
探讨结核性胸膜炎患者单核细胞氧化DNA损伤水平及总抗氧化能力（TAC），评估褪黑素对结核性胸膜炎患者胸腔积液中单核细胞DNA损伤的体外修复作用。
采用单细胞凝胶电泳（彗星实验）检测28例结核性胸膜炎患者胸腔积液及外周血中的单核细胞DNA损伤，以及25例健康人的外周血单核细胞DNA损伤。采用邻菲啰啉比色法测定28例患者胸腔积液上清液及血浆、25例健康人血浆中的TAC水平。体外给予20例患者胸腔积液中的单核细胞褪黑素处理。
结核性胸膜炎患者胸腔积液中单核细胞彗星实验百分比为（41.3±14.5）%，高于外周血中的（21.2±4.2）%，P<0.01。胸腔积液上清液中TAC水平为（5.17±1.19）U/ml，低于血浆中的（8.66±1.59）U/ml，P<0.01。胸腔积液中单核细胞彗星实验百分比与TAC水平呈负相关（r=-0.425，P<0.05）。患者外周血单核细胞彗星实验百分比为（21.2±4.2）%，高于对照组的（8.9±3.7）%，P<0.01。血浆中TAC水平为（8.66±1.59）U/ml，低于对照组的（10.61±1.39）U/ml，P<0.01。褪黑素处理后，胸腔积液中单核细胞彗星实验百分比从（40.8±9.3）%降至（11.0±3.7）%，P<0.01。
结核性胸膜炎患者存在氧化DNA损伤及氧化/抗氧化失衡，尤其在患病胸腔。褪黑素可能通过其抗氧化作用促进结核性胸膜炎患者胸腔积液中单核细胞受损DNA的体外修复。
