Licciano Margherita, Terlizzi Antonio, Giangrande Adriana, Cavallo Rosa Anna, Stabili Loredana
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (DiSTeBA), Via Prov. Lecce-Monteroni, 73100 Lecce, Italy.
Mar Environ Res. 2007 Oct;64(4):504-13. doi: 10.1016/j.marenvres.2007.04.004. Epub 2007 Apr 27.
The present study investigates the effect of the filtering activity of Sabella spallanzanii on the culturable heterotrophic bacterial community through the comparison of the bacterial diversity in transplanted polychaetes and the surrounding seawater. For isolation of culturable heterotrophic bacteria, seawater samples as well as polychaete homogenates were plated in triplicates onto Bacto Marine Agar 2216 (Difco). All the colonies grown were isolated, subcultured and identified by several morphological, biochemical and cultural methods. Some bacterial genera showed higher average abundances in polychaetes than in seawater (i.e. Lucibacterium and Photobacterium). Aeromonas represented a conspicuous component of the bacterial community both in S. spallanzanii and seawater. The presence of Cytophaga and Pseudomonas was also relevant in the examined seawater samples. The selective concentrations of some bacterial genera inside S. spallanzanii either by grazing on bacteria or their capability as bacterial reservoirs, provides evidence for the role of macrobenthic invertebrates as key determinants for microbial diversity.
本研究通过比较移植多毛类动物及其周围海水中的细菌多样性，研究了萨氏沙蠋的过滤活动对可培养异养细菌群落的影响。为了分离可培养的异养细菌，将海水样本以及多毛类动物匀浆一式三份接种到细菌用海洋琼脂2216（Difco）上。所有生长的菌落均进行分离、传代培养，并通过多种形态学、生物化学和培养方法进行鉴定。一些细菌属在多毛类动物中的平均丰度高于海水中（即发光杆菌属和发光细菌属）。气单胞菌是萨氏沙蠋和海水中细菌群落的一个显著组成部分。噬纤维菌属和假单胞菌属在检测的海水样本中也很重要。萨氏沙蠋体内某些细菌属的选择性富集，无论是通过捕食细菌还是作为细菌储存库的能力，都为大型底栖无脊椎动物作为微生物多样性关键决定因素的作用提供了证据。
