Chalkias Nikolaos G, Giannelis Emmanuel P
School of Chemical and Biomolecular Engineering, Olin Hall 120, Cornell University, Ithaca, NY 14853, USA.
Biosens Bioelectron. 2007 Oct 31;23(3):370-6. doi: 10.1016/j.bios.2007.04.014. Epub 2007 May 1.
Since their introduction, artificial lipid bilayer membranes were used in a wide array of applications, such as sensors, biocompatible materials and study-models of the cell's outer boundary. Here, we present a nanohybrid membrane using an inorganic host and amphiphilic organic molecules with lipid bilayer-like properties. The stability of the presented mimetic membrane is significantly improved when compared to existing methods. The nanohybrid membrane exhibited two thermotropic phases corresponding to the L(alpha) and L(beta) phases that lipid bilayer membranes are known to adopt. Integration of cholesterol molecules into the nanohybrid membrane lead to the same qualitative effects as in lipid bilayers, including expansion of the bilayer spacing and decrease of the L(alpha) to L(beta) transition enthalpy. To further illustrate the similarities of the synthesized membrane with a lipid bilayer, the ability of the nanohybrid membrane to function as saccharin conductimetric sensor was evaluated. The lower limit of detection of the sensor was 6 microM and the linear range of response was from 20 to 400 microM.
自从人工脂质双分子层膜被引入以来，它们被广泛应用于各种领域，如传感器、生物相容性材料以及细胞外边界的研究模型。在此，我们展示一种使用无机主体和具有类似脂质双分子层性质的两亲性有机分子的纳米复合膜。与现有方法相比，所展示的模拟膜的稳定性得到了显著提高。该纳米复合膜呈现出两个对应于脂质双分子层膜已知的L(α)相和L(β)相的热致相。将胆固醇分子整合到纳米复合膜中会产生与脂质双分子层中相同的定性效果，包括双层间距的扩大和L(α)到L(β)转变焓的降低。为了进一步说明合成膜与脂质双分子层的相似性，评估了纳米复合膜作为糖精电导传感器的功能。该传感器的检测下限为6微摩尔，响应线性范围为20至400微摩尔。
