[薄层色谱法对17 - 氧类固醇的分组测定]
[Group determination of 17-oxosteroids on thin-layer chromotography].
作者信息
Tomsová Z, Jůzová Z
机构信息
III. interní klinika 1. lékarské fakulty Univerzity Karlovy, Praha.
出版信息
Sb Lek. 1991 Aug;93(7-8):232-8.
17-oxosteroids from human urine are measured by thin layer chromatography (Merck Kieselgel F-254 plates) with fluorometric detection at 310 nm (densitometer SHIMADZU CS-9000): 11-OH-etiocholanolone, 11-OH-androsterone + oxoetiocholanolone, oxoandrosterone, dehydroepiandrosterone and androsterone. Fluorescence of hydrazone derivatives after the reaction of steroids with dansylhydrazine represents the principle of the method. The recoveries of steroids added to human urine samples are 97-101.3% and the coefficients of variation are 0.78-11.77.
采用薄层色谱法（默克硅胶F - 254板），在310nm波长下进行荧光检测（岛津CS - 9000密度计），测定人尿中的17 - 氧代类固醇：11 - 羟基 - 本胆烷醇酮、11 - 羟基 - 雄甾酮+氧代本胆烷醇酮、氧代雄甾酮、脱氢表雄酮和雄甾酮。类固醇与丹磺酰肼反应后腙衍生物的荧光代表了该方法的原理。添加到人类尿液样本中的类固醇回收率为97 - 101.3%，变异系数为0.78 - 11.77。
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