Yang Jia-Da, Shang Hai-Tao, Wei Hong, Yang Wan-Shen, Liu Xin
Department of Biochemistry, College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China.
Yi Chuan. 2007 May;29(5):575-80. doi: 10.1360/yc-007-0575.
CYP3A29 is the most important key enzyme for drug metabolism in pig's liver. Research of the characters of CYP3A29 mRNA expression in Chinese experimental miniature pig's livers is significant for evaluating it whether suitable for experimental model for drug metabolism mediated by human CYP3A4 enzyme. In this study, the levels of CYP3A29 mRNA expression in livers of Bama miniature pigs, Guizhou miniature pigs and Rongchang pigs were studied by TaqMan-mediated quantitative RT-PCR. Results indicated that the CYP3A29 mRNA expression levels in livers of these species were close to literatures of human. Levels of CYP3A29 mRNA expression were similar among livers of Bama miniature pigs, Guizhou miniature pigs and Rongchang pigs, but interindividual variations were quite large. It was suggested that Bama miniature pigs and Guizhou miniature pigs were both feasible for experimental animal model for evaluating drug metabolism in some degree.
CYP3A29是猪肝中药物代谢最重要的关键酶。研究中国实验用小型猪肝脏中CYP3A29 mRNA表达特征，对于评估其是否适合作为人CYP3A4酶介导的药物代谢实验模型具有重要意义。本研究采用TaqMan介导的定量RT-PCR技术，研究了巴马小型猪、贵州小型猪和荣昌猪肝脏中CYP3A29 mRNA的表达水平。结果表明，这些猪种肝脏中CYP3A29 mRNA的表达水平与人类文献报道相近。巴马小型猪、贵州小型猪和荣昌猪肝脏中CYP3A29 mRNA的表达水平相似，但个体间差异较大。提示巴马小型猪和贵州小型猪在一定程度上均适用于评估药物代谢的实验动物模型。
