Bao Wen-Bin, Chen Guo-Hong, Wu Xin-Sheng, Xu Qi, Wu Sheng-Long, Shu Jing-Ting, Weigend Steffen
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yi Chuan. 2007 May;29(5):587-92. doi: 10.1360/yc-007-0587.
Genetic diversity of red jungle fowl in China (Gallus gallus spadiceus) and red jungle fowl in Thailand (Gallus gallus gallus) was evaluated with 29 microstaellite loci, the genetic variability within subspecies and genetic differentiation between subspecies were estimated. The results showed that the 168 alleles were amplified with the number of alleles per locus from 2 to 13. The average expected heterozygosity and polymorphism information content (PIC) of all loci were 0.5780 and 0.53, respectively. The mean numbers of effective alleles of red jungle fowl in China and red jungle fowl in Thailand were 5.55 and 6.38. The heterozygosity and the genetic diversity of the two subspecies were high. Genetic differentiation index (FST) of these populations was 0.194 (P<0.01). Reynolds' genetic distance and gene flow between the two populations were 0.157 and 1.040, respectively. Based on these results, genetic structure and significant genetic differentiation of red jungle fowl in China were different from red jungle fowl in Thailand. The results of this study did not support to identify these red jungle fowl subspecies as the same subspecies, but supported the theory that Chinese domestic fowls have independent origin.
利用29个微卫星位点对中国红色原鸡（Gallus gallus spadiceus）和泰国红色原鸡（Gallus gallus gallus）的遗传多样性进行了评估，估算了亚种内的遗传变异性和亚种间的遗传分化。结果显示，共扩增出168个等位基因，每个位点的等位基因数为2至13个。所有位点的平均期望杂合度和多态信息含量（PIC）分别为0.5780和0.53。中国红色原鸡和泰国红色原鸡的平均有效等位基因数分别为5.55和6.38。两个亚种的杂合度和遗传多样性都很高。这些群体的遗传分化指数（FST）为0.194（P<0.01）。两个群体之间的雷诺兹遗传距离和基因流分别为0.157和1.040。基于这些结果，中国红色原鸡与泰国红色原鸡的遗传结构和显著的遗传分化有所不同。本研究结果不支持将这些红色原鸡亚种鉴定为同一亚种，但支持中国家鸡具有独立起源的理论。
