Takenaka Shunsuke, Safroneeva Ekaterina, Xing Zhou, Gauldie Jack
Center for Gene Therapeutics, Department of Pathology and Molecular Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada.
J Immunol. 2007 Jun 15;178(12):7984-93. doi: 10.4049/jimmunol.178.12.7984.
Dendritic cells (DCs) residing in different tissues and exposed to different organisms are likely to have different reactivities to their surrounding environment. Many studies use in vitro generated DCs to examine functions of these cells, but such cells may not truly reflect the nature of DCs and their in situ activities in vivo. We have used magnetic label-based technique to isolate colonic DCs to conduct derailed characterization of these cells. Colonic DCs comprise mainly CD11b+ DCs with few CD8alpha+ DCs or plasmacytoid DCs. Functionally, isolated colonic DCs are able to endocytose and process proteins, undergo maturation, and stimulate T cells to proliferate. Importantly, expression of TLRs by colonic DCs is significantly lower than that of their spleen counterparts; however, they appear to be as, or more, responsive to stimulation by oligodeoxynucleotides containing CpG motif based on their cytokine production. We speculate that colonic DCs have unique reactivities differing from DCs residing in other lymphoid tissues and are adapted for the unique microenvironment of the colonic mucosa and that these cells react uniquely to their environment.
驻留在不同组织中并接触不同生物体的树突状细胞（DCs）可能对其周围环境具有不同的反应性。许多研究使用体外生成的DCs来研究这些细胞的功能，但此类细胞可能无法真实反映DCs的本质及其在体内的原位活性。我们已使用基于磁性标记的技术分离结肠DCs，以对这些细胞进行详细表征。结肠DCs主要由CD11b + DCs组成，只有少量CD8α + DCs或浆细胞样DCs。在功能上，分离出的结肠DCs能够内吞和处理蛋白质、进行成熟并刺激T细胞增殖。重要的是，结肠DCs的Toll样受体（TLRs）表达明显低于脾脏中的对应细胞；然而，基于其细胞因子产生情况，它们似乎对含CpG基序的寡脱氧核苷酸刺激具有相同或更强的反应性。我们推测，结肠DCs具有不同于驻留在其他淋巴组织中的DCs的独特反应性，并且适应于结肠黏膜的独特微环境，并且这些细胞对其环境有独特的反应。
