Yamanoshita Osamu, Ichihara Shu, Hama Hidetoshi, Ichihara Gaku, Chiba Momoko, Kamijima Michihiro, Takeda Iwao, Nakajima Tamie
Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Japan.
Tohoku J Exp Med. 2007 Jun;212(2):191-8. doi: 10.1620/tjem.212.191.
Breast cancer is one of the major cancers in women, and dietary intake must be controlled to prevent it. Selenium (Se), especially Se compound in vegetables, is thought to be a promising chemopreventive dietary ingredient for preventing breast cancer. In this study, we developed Se-enriched Japanese radish sprout using a special Se-additional fertilizer, and identified the Se chemical forms. The newly developed Se-enriched sprout is produced within a week by the tank forming method, and the major chemical form was identified as Se-methylselenocysteine (MeSeCys) (80%). Then, the chemopreventive effects of the Se-enriched sprout were investigated using Sprague-Dawley female rats with mammary cancer, induced by a single oral dose of 10 mg or 14 mg of 7, 12-dimethylbenz[a]anthracene (DMBA). Mammary tumors were found in 11, 16 and 2 rats treated with DMBA and thereafter fed the basal (n = 34), sprout-added basal (n = 30) and Se-enriched sprout-added test diets (n = 30), respectively. The incidence of mammary tumors was significantly lower in the Se-enriched sprout-added test diet group (7%) than in the basal diet group (32%) or sprout-added basal diet group (53%). In contrast, no significant difference was detected in the numbers and incidence of the tumor between the basal diet group and Se-enriched sprout-added test diet group before DMBA-dosing. These results suggest that the diet supplement of Se-enriched sprout after DMBA-dosing provides a significant chemoprevention against chemical-induced mammary cancer. Thus, Se-enriched sprout may be a useful dietary ingredient for preventing breast cancer.
乳腺癌是女性主要的癌症之一，必须控制饮食摄入以预防乳腺癌。硒（Se），尤其是蔬菜中的硒化合物，被认为是一种很有前景的预防乳腺癌的化学预防膳食成分。在本研究中，我们使用一种特殊的添加硒的肥料培育出富硒日本萝卜芽，并鉴定了其中硒的化学形态。新培育出的富硒芽通过水培法在一周内产出，其主要化学形态被鉴定为甲基硒代半胱氨酸（MeSeCys）（80%）。然后，我们使用单次口服10毫克或14毫克7,12-二甲基苯并[a]蒽（DMBA）诱导患乳腺癌的Sprague-Dawley雌性大鼠，研究了富硒芽的化学预防效果。在分别用DMBA处理后并喂食基础饲料（n = 34）、添加芽的基础饲料（n = 30）和添加富硒芽的试验饲料（n = 30）的大鼠中，分别有11只、16只和2只发现乳腺肿瘤。添加富硒芽的试验饲料组的乳腺肿瘤发生率（7%）显著低于基础饲料组（32%）或添加芽的基础饲料组（53%）。相比之下，在给予DMBA之前，基础饲料组和添加富硒芽的试验饲料组之间的肿瘤数量和发生率没有显著差异。这些结果表明，在给予DMBA后补充富硒芽的饮食可对化学诱导的乳腺癌提供显著的化学预防作用。因此，富硒芽可能是预防乳腺癌的一种有用的膳食成分。
