Chong Inn-Wen, Chang Mei-Yin, Sheu Chau-Chyun, Wang Cheng-Yuan, Hwang Jhi-Jhu, Huang Ming-Shyan, Lin Shiu-Ru
Division of Pulmonary and Critical Care Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan, R.O.C.
Oncol Rep. 2007 Jul;18(1):17-24.
The ability to detect K-ras oncogene may provide additional information for the management of patients with non-small cell lung cancer (NSCLC). In the present study, we detected the K-ras oncogene in 76 patients with NSCLC by two methods: direct sequencing of K-ras in tumor tissues and membrane array detection of the gene overexpression specific for activated K-ras in peripheral blood. The results showed that 28 (36.8%) of the 76 Taiwanese NSCLC patients had K-ras mutations, with a frequency of 36.4% (20/55) in adenocarcinomas and 38.1% (8/21) in squamous cell carcinomas. The K-ras mutations were more frequently found in smokers than in non-smokers (51.4 vs. 24.4%, P=0.015). The incidences of K-ras mutation in the subgroups of non-smokers and squamous cell carcinomas are relatively higher in Taiwan than in other countries. On the other hand, the membrane array method could positively detect circulating activated K-ras in all of the 27 NSCLC patients with K-ras mutations at codons 12, 13 and 61, and in 4 of the 48 patients with wild-type K-ras. Our results suggest that the K-ras oncogene membrane array serves as a sensitive and convenient tool for the detection of K-ras oncogene, and therefore, has a great potential for clinical applications.
检测K-ras癌基因的能力可能为非小细胞肺癌（NSCLC）患者的管理提供额外信息。在本研究中，我们通过两种方法检测了76例NSCLC患者的K-ras癌基因：肿瘤组织中K-ras的直接测序和外周血中活化K-ras特异性基因过表达的膜阵列检测。结果显示，76例台湾NSCLC患者中有28例（36.8%）发生K-ras突变，腺癌中的突变频率为36.4%（20/55），鳞状细胞癌中的突变频率为38.1%（8/21）。K-ras突变在吸烟者中比在非吸烟者中更常见（51.4%对24.4%，P=0.015）。台湾非吸烟者和鳞状细胞癌亚组中K-ras突变的发生率相对高于其他国家。另一方面，膜阵列方法可以在所有27例密码子12、13和61处发生K-ras突变的NSCLC患者以及48例野生型K-ras患者中的4例中阳性检测到循环活化K-ras。我们的结果表明，K-ras癌基因膜阵列是检测K-ras癌基因的一种灵敏且便捷的工具，因此具有很大的临床应用潜力。
