Endres Michaela, Neumann Katja, Häupl Thomas, Erggelet Christoph, Ringe Jochen, Sittinger Michael, Kaps Christian
TransTissue Technologies GmbH, Tucholskystrasse 2, 10117 Berlin, Germany.
J Orthop Res. 2007 Oct;25(10):1299-307. doi: 10.1002/jor.20394.
Microfracture is a frequently used reparative technique that induces a healing response in articular cartilage defects. Penetration of the subchondral bone leads to blood clot formation, allows multipotent mesenchymal cells to access the defect and, subsequently, leads to cartilaginous repair tissue. The aim of our study was to analyze the chemotactic recruitment of human subchondral spongious bone marrow-derived cells by synovial fluid (SF) from normal donors (ND), patients with osteoarthritis (OA) and rheumatoid arthritis (RA). Subchondral spongious bone marrow-derived mesenchymal progenitors were isolated from bone cylinders after high tibial osteotomy and analyzed for the presence of stem cell-related cell surface antigens by flow cytometry. Recruitment of subchondral progenitors by normal SF and SF from donors with degenerated joint diseases was documented by using a modified Boyden chamber chemotaxis assay. The chemotaxis assay demonstrated that synovial fluid has the potential to recruit mesenchymal progenitors in vitro. SF from normal donors and patients with OA showed no difference in the potential to stimulate cell migration. SF obtained from RA donors showed significantly reduced cell recruitment compared to SF derived from OA patients (p = 0.0054) and normal donors (p < 0.0001). The chemotactic activity of SF obtained from normal donors and from patients with degenerative joint diseases suggests that SF may be actively involved in the migration of progenitors in cartilage defects after microfracture.
微骨折术是一种常用的修复技术，可诱导关节软骨缺损处产生愈合反应。穿透软骨下骨会导致血凝块形成，使多能间充质细胞进入缺损处，随后形成软骨修复组织。我们研究的目的是分析来自正常供体（ND）、骨关节炎（OA）患者和类风湿关节炎（RA）患者的滑液（SF）对人软骨下松质骨髓来源细胞的趋化募集作用。在高位胫骨截骨术后，从骨圆柱体中分离出软骨下松质骨髓来源的间充质祖细胞，并通过流式细胞术分析干细胞相关细胞表面抗原的存在情况。使用改良的Boyden小室趋化试验记录正常SF和来自关节疾病退变供体的SF对软骨下祖细胞的募集情况。趋化试验表明，滑液在体外具有募集间充质祖细胞的潜力。来自正常供体和OA患者的SF在刺激细胞迁移的潜力方面没有差异。与来自OA患者（p = 0.0054）和正常供体（p < 0.0001）的SF相比，来自RA供体的SF显示细胞募集明显减少。来自正常供体和关节疾病退变患者的SF的趋化活性表明，SF可能积极参与微骨折术后软骨缺损处祖细胞的迁移。
