Ma X F, Gibbons J A, Babish J G
Department of Pharmacology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Toxicol Lett. 1991 Dec;59(1-3):51-8. doi: 10.1016/0378-4274(91)90054-a.
Hepatic microsomes were prepared from immature C57BL/6J mice 24 h after receiving intraperitoneal injections of either corn oil, benzo[e]pyrene (BeP, 50 mg/kg) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 4 x 10(-3) mg/kg). The capacity of these hepatic microsomes to bioactivate aflatoxin B1 (AFB1), 2-aminoanthracene (AA), benzo[a]pyrene (BaP), 3-methylcholanthrene (MC), 7,12-dimethylbenzanthracene (DMBA), BeP and pyrene (PY) was measured using strain TA100 in the Salmonella typhimurium/microsome reversion assay. BeP pretreatment of mice resulted in a 33% increase in mutagenic potency (MP) of AFB1 over the corn oil controls and a 70% increase in MP relative to TCDD-pretreated microsomes. With AA, BaP and DMBA as promutagens, BeP pretreatment reduced MP an average of 24%, while TCDD pretreatment increased MP of these 3 promutagens 263% compared to controls. Since the general effects of BeP and TCDD on murine hepatic cytochrome P-450 (P450)-mediated activities in this study were discordant, it appears that changes in P450 activity by BeP pretreatment are not mediated through the Ah receptor.
在给未成熟的C57BL/6J小鼠腹腔注射玉米油、苯并[e]芘（BeP，50毫克/千克）或2,3,7,8-四氯二苯并对二恶英（TCDD，4×10⁻³毫克/千克）24小时后，制备肝微粒体。使用鼠伤寒沙门氏菌/微粒体回复突变试验中的TA100菌株，测定这些肝微粒体对黄曲霉毒素B1（AFB1）、2-氨基蒽（AA）、苯并[a]芘（BaP）、3-甲基胆蒽（MC）、7,12-二甲基苯并蒽（DMBA）、BeP和芘（PY）的生物活化能力。用BeP预处理小鼠后，与玉米油对照组相比，AFB1的诱变效力（MP）增加了33%，相对于用TCDD预处理的微粒体，MP增加了70%。以AA、BaP和DMBA作为前诱变剂时，BeP预处理使MP平均降低了24%，而TCDD预处理使这3种前诱变剂的MP相对于对照组增加了263%。由于在本研究中BeP和TCDD对小鼠肝细胞色素P-450（P450）介导的活性的总体影响不一致，因此看来BeP预处理引起的P450活性变化不是通过芳烃受体介导的。
