Putranto Heri Dwi, Kusuda Satoshi, Inagaki Kayo, Kumagai Gaku, Ishii-Tamura Rie, Uziie Yoko, Doi Osamu
Laboratory of Animal Reproduction, United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Japan.
J Vet Med Sci. 2007 May;69(5):569-71. doi: 10.1292/jvms.69.569.
Feces were collected from two female and one male Siberian tigers, Panthera tigris altaica. Steroid hormones were extracted from lyophilized feces and quantified by enzyme immunoassay. The fecal contents of estradiol-17beta (E(2)) and testosterone in the females and male, respectively, changed markedly throughout the year. The fecal E(2) contents of females Nos. 179 and 238 increased at 26.4 +/- 8.0 and 28.0 +/- 14.2 day intervals, respectively. However, the fecal contents of progesterone (P(4)) in the female kept alone did not change. In contrast, the other female, which was kept with a male, had increased fecal P(4) contents after copulation. The fecal progesterone levels of the pregnant female remained high during her 106-day pregnancy.
从两只雌性和一只雄性东北虎（Panthera tigris altaica）采集粪便。从冻干粪便中提取类固醇激素，并通过酶免疫测定法定量。雌性和雄性粪便中雌二醇 - 17β（E₂）和睾酮的含量全年分别有显著变化。179号和238号雌性的粪便E₂含量分别以26.4±8.0天和28.0±14.2天的间隔增加。然而，单独饲养的雌性粪便中孕酮（P₄）含量没有变化。相比之下，与雄性一起饲养的另一只雌性在交配后粪便P₄含量增加。怀孕雌性在106天的孕期内粪便孕酮水平一直很高。
