Timmons Brian W, Bar-Or Oded
Children's Exercise and Nutrition Centre, Departments of Pediatrics, McMaster University, Hamilton, ON, Canada.
Eur J Appl Physiol. 2007 Sep;101(2):233-40. doi: 10.1007/s00421-007-0495-7. Epub 2007 Jun 6.
Distinct natural killer (NK) cell subsets (CD56bright and CD56dim) are mobilized with exercise and these cells may serve adaptive functions. We determined the distribution of NK cell subsets in response to exercise and carbohydrate (CHO) intake in young girls and compared these responses with previous findings in young boys of the same age. Twelve girls (12 years old) cycled for 60 min at 70% VO2max while drinking 6% CHO or flavoured water. Blood was collected at rest, during (30 and 60 min) and following (30 and 60 min) exercise to identify NK cells as CD3-CD56bright or CD3-CD56dim. CD69 expression on total CD3-CD56+ cells was also determined. A trend (P=0.07) was found for a trial x time interaction in CD56dim cell counts, with values lower with CHO than with water. CHO intake did not influence CD56bright responses (P>or=0.39). The CD56bright:CD56dim ratio increased during recovery from exercise (P<0.001), compared to rest, with no effect of CHO intake (P=0.48). CD69 expression was not different between exercise or recovery and rest. Like young boys, girls experience an elevated CD56bright:CD56dim ratio during recovery from exercise and CHO intake attenuates the exercise-induced CD56dim but not CD56bright cell response. Unlike young boys, girls do not experience a CHO-induced increase in the CD56bright:CD56dim ratio during recovery and CD69 expression does not increase on CD3-CD56+ cells during recovery. We conclude that even in young children sex-based differences exist in the NK cell response to exercise and CHO intake.
不同的自然杀伤（NK）细胞亚群（CD56bright和CD56dim）会因运动而被动员起来，这些细胞可能发挥适应性功能。我们确定了年轻女孩运动及摄入碳水化合物（CHO）后NK细胞亚群的分布，并将这些反应与同年龄年轻男孩之前的研究结果进行了比较。12名12岁女孩在饮用6% CHO或调味水的同时，以70%最大摄氧量进行60分钟的骑行。在静息状态、运动期间（30分钟和60分钟）以及运动后（30分钟和60分钟）采集血液，以鉴定NK细胞为CD3-CD56bright或CD3-CD56dim。还测定了总CD3-CD56+细胞上CD69的表达。在CD56dim细胞计数中发现了试验×时间交互作用的趋势（P=0.07），CHO组的值低于水组。CHO摄入不影响CD56bright反应（P≥0.39）。与静息状态相比，运动恢复期间CD56bright:CD56dim比值增加（P<0.001），CHO摄入无影响（P=0.48）。运动或恢复期间与静息状态下CD69表达无差异。与年轻男孩一样，女孩在运动恢复期间CD56bright:CD56dim比值升高，CHO摄入减弱运动诱导的CD56dim细胞反应，但不影响CD56bright细胞反应。与年轻男孩不同的是，女孩在恢复期间未出现CHO诱导的CD56bright:CD56dim比值增加，且恢复期间CD3-CD56+细胞上CD69表达未增加。我们得出结论，即使在幼儿中，NK细胞对运动和CHO摄入的反应也存在基于性别的差异。
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