Song Xing-Hua, Xu Xiao-Xiong, Ding Li-Wen, Cao Li, Sadel Alken, Wen Hao
Department of Orthopaedics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uighur Autonomous Region, China.
World J Gastroenterol. 2007 May 14;13(18):2575-80. doi: 10.3748/wjg.v13.i18.2575.
To determine the expression of c-Fos, caspase-3 and interleukin-1beta (IL-1beta) in the cervical cord and stomach of rats with cervical spondylosis, to analyze their relationship, and to offer an explanation of one possible cause for functional dyspepsia (FD) and irritable bowel syndrome (IBS) caused by cervical spondylosis.
The cervical spondylosis model in rats was established by destroying the stability of cervical posterior column. The cord segments C4-6 and gastric antrum were collected 3 mo and 5 mo after the operation. Rats with the sham operation were used as controls. The expressions of c-Fos, caspase-3 and IL-1beta in the cervical cord and gastric antrum were determined by immunohistochemistry and/or Western blot.
Immunohistochemical staining showed a few c-Fos, caspase-3 and IL-1beta-positive cells in the cervical cord and antrum in the control. There was a significant increase in c-Fos, caspase-3 and IL-1beta expression in model groups compared to the control groups at 3 mo and 5 mo after operation. More importantly, there was a significant (P < 0.05) increase in c-Fos, caspase-3 and IL-1beta expression in the model group rats at 3 mo compared to those at 5 mo after the operation (c-Fos: 11.20 +/- 2.26 vs 27.68 +/- 4.36 in the cervical cord, 11.3 +/- 2.3 vs 29.3 +/- 4.6 in the gastric antrum; caspase-3: 33.83 +/- 3.71 vs 36.32 +/- 4.01 in the cervical cord, 13.23 +/- 3.21 vs 26.32 +/- 4.01 in the gastric antrum; IL-1beta: 42.06 +/- 2.95 vs 45.91 +/- 3.98 in the cervical cord, 26.56 +/- 2.65 vs 32.01 +/- 2.98 in the gastric antrum). Western blot analysis showed time-dependent changes of caspase-3 and IL-1beta protein in the cervical cord and gastric antrum of rats with cervical spondylosis; there was no significant expression of caspase-3 and IL-1beta protein in the control group at 3 mo and 5 mo after the sham operation, whereas there was a significant difference in caspase-3 and IL-1beta protein levels between the model group rats followed up for 3 mo and those for 5 mo (P < 0.05).
There is a significant association of c-Fos, caspase-3 and IL-1beta expressions in the gastric antrum with that in the spinal cord in rats with cervical spondylosis, suggesting that the gastrointestinal function may be affected by cervical spondylosis.
测定颈椎病大鼠颈髓和胃中c-Fos、半胱天冬酶-3（caspase-3）和白细胞介素-1β（IL-1β）的表达，分析它们之间的关系，并为颈椎病引起的功能性消化不良（FD）和肠易激综合征（IBS）的一种可能原因提供解释。
通过破坏颈后柱稳定性建立大鼠颈椎病模型。术后3个月和5个月收集颈4-6节段脊髓和胃窦。假手术大鼠作为对照。采用免疫组织化学和/或蛋白质印迹法测定颈髓和胃窦中c-Fos、caspase-3和IL-1β的表达。
免疫组织化学染色显示，对照组颈髓和胃窦中有少量c-Fos、caspase-3和IL-1β阳性细胞。与对照组相比，模型组术后3个月和5个月时c-Fos、caspase-3和IL-1β表达显著增加。更重要的是，与术后5个月相比，模型组大鼠术后3个月时c-Fos、caspase-3和IL-1β表达显著升高（P<0.05）（颈髓中c-Fos：11.20±2.26对27.68±4.36，胃窦中11.3±2.3对29.3±4.6；caspase-3：颈髓中33.83±3.71对36.32±4.01，胃窦中13.23±3.21对26.32±4.01；IL-1β：颈髓中42.06±2.95对45.91±3.98，胃窦中26.56±2.65对32.01±2.98）。蛋白质印迹分析显示，颈椎病大鼠颈髓和胃窦中caspase-3和IL-1β蛋白呈时间依赖性变化；假手术组术后3个月和5个月时对照组无caspase-3和IL-1β蛋白显著表达，而模型组随访3个月和5个月的大鼠caspase-3和IL-1β蛋白水平有显著差异（P<0.05）。
颈椎病大鼠胃窦中c-Fos、caspase-3和IL-1β表达与脊髓中的表达显著相关，提示颈椎病可能影响胃肠功能。
