Feng Yi, Tian Zhong-Min, Wan Ming-Xi, Zheng Zhao-Bin
Department of Biomedical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
World J Gastroenterol. 2007 May 14;13(18):2608-14. doi: 10.3748/wjg.v13.i18.2608.
To investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy.
Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis (2DE). The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.
We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.
Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.
研究人肝癌细胞系SMMC - 7721的蛋白质谱，分析高表达蛋白在肝癌发生、生长和转移过程中的特定功能，鉴定用于早期诊断预测的肝癌特异性生物标志物，并探索肝癌治疗的新药物靶点。
采用双向电泳（2DE）分离人肝癌细胞系SMMC - 7721的总蛋白。用2DE软件Image Master 2D Elite分析银染凝胶。基于基质辅助激光解吸/电离飞行时间质谱（MALDI - TOF - MS）和数据库搜索，通过肽质量指纹图谱鉴定感兴趣的蛋白点。
我们获得了人肝癌细胞系SMMC - 7721的蛋白质谱。在成功鉴定的21种蛋白质中，线粒体丝状蛋白、内质网蛋白ERp29、泛醌 - 细胞色素C还原酶复合体核心蛋白I、过氧化物酶体烯酰辅酶A水合酶、过氧化物还原蛋白 - 4和可能的3 - 氧代酸辅酶A转移酶1前体是在人肝癌细胞或组织中鉴定出的6种新蛋白质。详细分析了已鉴定的热休克蛋白的特定功能，结果表明这些蛋白可能通过抑制几种癌症相关应激诱导的细胞死亡或通过在凋亡信号通路的多个点抑制凋亡来促进肿瘤发生。还分析了其他已鉴定的伴侣蛋白和癌症相关蛋白。
基于SMMC - 7721细胞的蛋白质谱，功能分析表明已鉴定的伴侣蛋白和癌症相关蛋白在肝癌的肿瘤发生、肿瘤生长和转移中各自有其作用途径。此外，蛋白质组学分析在癌症研究中被证明是可行的。
