Ohmori T, Takamoto K, Ogata M
Department of Public Health, Okayama University Medical School, Japan.
Acta Med Okayama. 1991 Oct;45(5):321-4. doi: 10.18926/AMO/32202.
Some mechanisms to reduce methemoglobin (metHb) formation for the maintenance of normal oxygen transport have been proposed. To study the role of catalase (EC 1.11.1.6), metHb formation in the hemolysate of normal and Japanese acatalasemic human subjects were examined spectrophotometrically. Significantly increased level of metHb was induced by potassium ferrocyanide in the hemolysate of acatalasemic subject. The addition of catalase reduced the metHb formation, while 3-amino-1,2,4-triazole (AT), a specific inhibitor of catalase-H2O2 compound I, increased it. These results obtained from human subjects were well consistent with those from mice and suggested that catalase plays a role in protecting erythrocytes against metHb formation.
已经提出了一些减少高铁血红蛋白（metHb）形成以维持正常氧运输的机制。为了研究过氧化氢酶（EC 1.11.1.6）的作用，采用分光光度法检测了正常人和日本无过氧化氢酶血症患者溶血产物中高铁血红蛋白的形成。高铁氰化钾在无过氧化氢酶血症患者的溶血产物中诱导高铁血红蛋白水平显著升高。添加过氧化氢酶可减少高铁血红蛋白的形成，而过氧化氢酶-H2O2复合物I的特异性抑制剂3-氨基-1,2,4-三唑（AT）则会增加高铁血红蛋白的形成。从人类受试者获得的这些结果与从小鼠获得的结果非常一致，表明过氧化氢酶在保护红细胞免受高铁血红蛋白形成方面发挥作用。
