痘苗病毒基因F3L编码一种影响天然免疫反应的细胞内蛋白。
Vaccinia virus gene F3L encodes an intracellular protein that affects the innate immune response.
作者信息
Froggatt Graham C, Smith Geoffrey L, Beard Philippa M
机构信息
Department of Virology, Faculty of Medicine, Imperial College London, St Mary's Campus, Norfolk Place, London W2 1PG, UK.
出版信息
J Gen Virol. 2007 Jul;88(Pt 7):1917-1921. doi: 10.1099/vir.0.82815-0.
Abstract
The Vaccinia virus BTB/kelch protein F3 has been characterized and its effects on virus replication in vitro and virus virulence in vivo have been determined. The loss of the F3L gene had no effect on virus growth, plaque phenotype or cytopathic effect in cell culture under the conditions tested. However, the virulence of a virus lacking F3L in an intradermal model was reduced compared with controls, and this was demonstrated by a significantly smaller lesion and alterations to the innate immune response to infection. The predicted molecular mass of the F3 protein is 56 kDa; however, immunoblotting of infected cell lysates using an antibody directed against recombinant F3 revealed two proteins of estimated sizes 37 and 25 kDa.
摘要
痘苗病毒BTB/kelch蛋白F3已被鉴定，并且其对体外病毒复制和体内病毒毒力的影响也已确定。在测试条件下，F3L基因的缺失对细胞培养中的病毒生长、空斑表型或细胞病变效应没有影响。然而，在皮内模型中，缺乏F3L的病毒的毒力与对照相比有所降低，这通过明显更小的损伤以及对感染的先天免疫反应的改变得以证明。F3蛋白的预测分子量为56 kDa；然而，使用针对重组F3的抗体对感染细胞裂解物进行免疫印迹显示出两种估计大小分别为37 kDa和25 kDa的蛋白质。
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