嗜热栖热菌OT3来源的PH1010（古菌DUF54蛋白家族成员）的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins.
作者信息
Shirokane Michio, Sawano Yoriko, Miyazono Ken Ichi, Nagata Koji, Tanokura Masaru
机构信息
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):532-4. doi: 10.1107/S1744309107024487. Epub 2007 May 31.
Abstract
PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 A resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 A. The crystal contained two PH1010 molecules in the asymmetric unit (V(M) = 2.4 A(3) Da(-1)) and had a solvent content of 48%.
摘要
来自嗜热栖热菌OT3的PH1010，属于古菌DUF54蛋白家族成员，已被表达、纯化并结晶。使用聚乙二醇3350作为沉淀剂，通过坐滴气相扩散法进行结晶。利用同步辐射源，晶体的X射线衍射分辨率达到1.90 Å。晶体的空间群确定为P2(1)2(1)2(1)，晶胞参数a = 46.9、b = 49.5、c = 132.7 Å。不对称单元中晶体包含两个PH1010分子（V(M)=2.4 ų Da⁻¹），溶剂含量为48%。
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