Lacosta Ana Ma, Canudas Jesus, Gonzalez Cristina, Muniesa Pedro, Sarasa Manuel, Dominguez Luis
Laboratory of Neurobiology, Department of Anatomy, Embryology and Animal Genetics, Faculty of Veterinary, University of Zaragoza, Spain.
Int J Dev Biol. 2007;51(4):327-31. doi: 10.1387/ijdb.062217al.
Using immunostaining during early zebrafish embryogenesis, we report that the cranial and trunk neural crest expresses the paired box protein Pax7, thus revealing a novel neural crest marker in zebrafish. In the head, we show that Pax7 is broadly expressed in the cranial crest cells, which indicates that duplication of the paralogous group Pax3/7 at the origin of vertebrates included the conserved expression of Pax7 in the head neural crest of all of the vertebrates species studied so far. In the trunk, Pax7 recognizes both premigratory and migratory neural crest cells. Notably, we observed the expression of Pax7 during the development of melanophore, xanthophore and iridophore precursor cells. In contrast to the case of melanocyte precursors in birds, Pax7 showed overlapping expression with early melanin pigment. Finally, during the larva to adult transition, we show that pigment stem cells recapitulate the expression of Pax7.
通过在斑马鱼胚胎发育早期进行免疫染色，我们发现颅部和躯干神经嵴表达配对盒蛋白Pax7，从而揭示了斑马鱼中一种新的神经嵴标志物。在头部，我们发现Pax7在颅嵴细胞中广泛表达，这表明在脊椎动物起源时旁系同源基因Pax3/7的复制包括了到目前为止所研究的所有脊椎动物物种头部神经嵴中Pax7的保守表达。在躯干中，Pax7识别迁移前和迁移中的神经嵴细胞。值得注意的是，我们在黑素细胞、黄色素细胞和虹彩细胞前体细胞的发育过程中观察到了Pax7的表达。与鸟类黑素细胞前体的情况不同，Pax7与早期黑色素色素呈重叠表达。最后，在幼体到成体的转变过程中，我们发现色素干细胞重现了Pax7的表达。
