TLR2基因的Arg753Gly、TLR4基因的Asp299Gly和Thr399Ile基因多态性与土耳其人群的慢性牙周炎无关。
TLR2 Arg753Gly, TLR4 Asp299Gly and Thr399Ile gene polymorphisms are not associated with chronic periodontitis in a Turkish population.
作者信息
Berdeli Afig, Emingil Gülnur, Han Saygan Buket, Gürkan Ali, Atilla Gül, Köse Timur, Baylas Haluk
机构信息
Molecular Medicine Laboratory, Department of Pediatrics, School of Medicine, Ege University, Izmir, Turkey.
出版信息
J Clin Periodontol. 2007 Jul;34(7):551-7. doi: 10.1111/j.1600-051X.2007.01092.x.
AIM
Toll-like receptor (TLR) gene polymorphisms could affect the host's ability to respond to microbial pathogens. In this case-control study, the association of TLR2 and TLR4 gene polymorphisms with chronic periodontitis (CP) was investigated.
MATERIALS AND METHODS
Genomic DNA was obtained from the peripheral blood of 83 patients with CP and 106 periodontally healthy subjects. The TLR2 Arg753Gly, Arg677Trp and TLR4 Asp299Gly, Thr399Ile gene polymorphisms were genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. The data were analysed by a chi2 test, logistic regression analysis and the Mann-Whitney U test.
RESULTS
The 753Gln allele was found in 6.1% of the CP patients as compared with 6.6% in the control group (p>0.05). The frequency of the 299Gly and 399Ile allele was 2.4% and 1.8% in CP patients. For the healthy subjects, the frequency was 2.8% for the 299Gly and 2.5% for the 399Ile allele (p>0.05). None of the CP patients or healthy subjects showed homozygosity for the TLR2 and TLR4 alleles. Percentage of sites with bleeding on probing and plaque were significantly higher in 299Gly-positive patients compared with 299Gly-negative patients (p<0.05).
CONCLUSION
These results showed that the TLR2 and TLR4 gene polymorphisms studied are not associated with susceptibility to CP in Turkish patients.
目的
Toll样受体（TLR）基因多态性可能影响宿主对微生物病原体的反应能力。在这项病例对照研究中，研究了TLR2和TLR4基因多态性与慢性牙周炎（CP）的关联。
材料与方法
从83例CP患者和106例牙周健康受试者的外周血中获取基因组DNA。采用聚合酶链反应-限制性片段长度多态性方法对TLR2 Arg753Gly、Arg677Trp和TLR4 Asp299Gly、Thr399Ile基因多态性进行基因分型。数据采用卡方检验、逻辑回归分析和曼-惠特尼U检验进行分析。
结果
CP患者中753Gln等位基因的检出率为6.1%，对照组为6.6%（p>0.05）。CP患者中299Gly和399Ile等位基因的频率分别为2.4%和1.8%。健康受试者中，299Gly等位基因频率为2.8%，399Ile等位基因频率为2.5%（p>0.05）。CP患者和健康受试者均未显示TLR2和TLR4等位基因的纯合性。与299Gly阴性患者相比，299Gly阳性患者的探诊出血部位和菌斑百分比显著更高（p<0.05）。
结论
这些结果表明，所研究的TLR2和TLR4基因多态性与土耳其患者CP的易感性无关。
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