基于冷冻电子显微镜技术对肾脏钠钾ATP酶α亚基与β和γ亚基的关联研究
Association of renal Na,K-ATPase alpha-subunit with the beta- and gamma-subunits based on cryoelectron microscopy.
作者信息
Purhonen P, Thomsen K, Maunsbach A B, Hebert H
机构信息
Department of Biosciences and Nutrition and School of Technology and Health, Karolinska Institutet, Royal Institute of Technology, S-141 57, Huddinge, Sweden.
出版信息
J Membr Biol. 2006;214(3):139-46. doi: 10.1007/s00232-006-0056-8. Epub 2007 Jun 6.
Na,K-ATPase transports Na(+) and K(+) across cell membranes and consists of alpha- and beta-subunits. Na,K-ATPase also associates with small FXYD proteins that regulate the activity of the pump. We have used cryoelectron microscopy of two-dimensional crystals including data to 8 A resolution to determine the three-dimensional (3-D) structure of renal Na,K-ATPase containing FXYD2, the gamma-subunit. A homology model for the alpha-subunit was calculated from a Ca(2+)-ATPase structure and used to locate the additional beta- and gamma-subunits present in the 3-D map of Na,K-ATPase. Based on the 3-D map, the beta-subunit is located close to transmembrane helices M8 and M10 and the gamma-subunit is adjacent to helices M2 and M9 of the alpha-subunit.
钠钾ATP酶跨细胞膜转运钠（Na⁺）和钾（K⁺），由α和β亚基组成。钠钾ATP酶还与调节该泵活性的小FXYD蛋白相关联。我们利用二维晶体的冷冻电子显微镜技术（包括分辨率达8埃的数据）来确定含有γ亚基FXYD2的肾钠钾ATP酶的三维（3-D）结构。从钙（Ca²⁺）ATP酶结构计算出α亚基的同源模型，并用于定位钠钾ATP酶三维图谱中存在的额外β和γ亚基。基于该三维图谱，β亚基位于靠近跨膜螺旋M8和M10处，γ亚基与α亚基的螺旋M2和M9相邻。
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