Fujino Masayuki, Yamakami Kazuo, Oda Takashi, Omasu Fumihiro, Murai Teiko, Yoshizawa Nobuyuki
Department of Public Health, National Defense Medical College, Saitama - Japan.
J Nephrol. 2007 May-Jun;20(3):364-9.
The relation of nephritis-associated plasmin receptor (NAPlr) as a nephritogenic antigen in group A streptococci (GAS), to acute poststreptococcal glomerulonephritis (APSGN) and the potential of specific strains to cause APSGN are not fully understood. It would be helpful to determine whether certain GAS strains from APSGN patients specifically express NAPlr and whether strains from non-APSGN patients express lower levels or an altered form of NAPlr.
The sequence and levels of expression of NAPlr were assayed for strains of GAS isolated from patients with APSGN, pharyngitis, scarlet fever or toxic shock-like syndrome. Findings were evaluated with respect to naplr gene sequence, expression level of NAPlr, serotype and disease type.
In GAS strains from both APSGN and non-APSGN patients, the naplr gene showed few or no nucleotide alterations, and both types of GAS strains expressed NAPlr in vitro. There were no obvious differences in naplr gene sequence, expression of NAPlr, serotype or disease type between the GAS strains. In addition, groups C and G streptococci also carried a conserved naplr gene and expressed NAPlr in vitro.
These groups of streptococci that express NAPlr should be associated with APSGN, and this association may be independent of serotype or disease type.
作为A组链球菌（GAS）中致肾炎抗原的肾炎相关纤溶酶受体（NAPlr）与急性链球菌感染后肾小球肾炎（APSGN）的关系，以及特定菌株导致APSGN的可能性尚未完全明确。确定来自APSGN患者的某些GAS菌株是否特异性表达NAPlr，以及来自非APSGN患者的菌株是否表达较低水平或改变形式的NAPlr将会有所帮助。
对从APSGN、咽炎、猩红热或中毒性休克样综合征患者分离出的GAS菌株进行NAPlr的序列和表达水平检测。根据naplr基因序列、NAPlr表达水平、血清型和疾病类型对结果进行评估。
在来自APSGN和非APSGN患者的GAS菌株中，naplr基因几乎没有或没有核苷酸改变，并且两种类型的GAS菌株在体外均表达NAPlr。GAS菌株之间在naplr基因序列、NAPlr表达、血清型或疾病类型方面没有明显差异。此外，C组和G组链球菌也携带保守的naplr基因并在体外表达NAPlr。
这些表达NAPlr的链球菌组应与APSGN相关，并且这种关联可能独立于血清型或疾病类型。
