可变数目串联重复序列（VNTR）等位基因的概念：三种方法获得的载脂蛋白B 3'高变区基因分型结果比较

Concept of VNTR alleles: comparison of apolipoprotein B 3' hypervariable region genotyping results obtained by three methods.

作者信息

Heliö T

机构信息

University of Helsinki, First Department of Medicine, Finland.

出版信息

Biochem Biophys Res Commun. 1991 Dec 16;181(2):846-51. doi: 10.1016/0006-291x(91)91267-g.

DOI:10.1016/0006-291x(91)91267-g

PMID:1755863

Abstract

In this study the apolipoprotein B 3' hypervariable region genotyping results obtained by three polymerase chain reaction and gel electrophoresis -based methods were compared. In 43 subjects of Finnish origin three individuals had each one allele (3.5%) which exhibited different migration when the apolipoprotein B 3' hypervariable region amplification products were separated on nondenaturing polyacrylamide gel electrophoresis as compared to agarose or denaturing polyacrylamide gels. Segregation of the variantly migrating alleles could be demonstrated in pedigrees suggesting that the variation might be due to differences in the DNA sequence among alleles with same apparent size on agarose or denaturing polyacrylamide gels.

摘要

在本研究中，对通过三种基于聚合酶链反应和凝胶电泳的方法获得的载脂蛋白B 3'高变区基因分型结果进行了比较。在43名芬兰裔受试者中，有三人各有一个等位基因（3.5%），当载脂蛋白B 3'高变区扩增产物在非变性聚丙烯酰胺凝胶上分离时，与琼脂糖或变性聚丙烯酰胺凝胶相比，该等位基因表现出不同的迁移情况。在系谱中可以证明变异迁移等位基因的分离，这表明该变异可能是由于在琼脂糖或变性聚丙烯酰胺凝胶上具有相同表观大小的等位基因之间的DNA序列差异所致。