Glisic S, Sunjevaric I, Alavantic D
Laboratory for Radiobiology and Molecular Genetics, Vinca Institute of Nuclear Sciences, Belgrade, Yugoslavia.
Electrophoresis. 1995 Jun;16(6):899-902. doi: 10.1002/elps.11501601151.
Three different ways of detecting insertion/deletion (Ins/Del) genotypes of the apolipoprotein B gene encoding signal peptide were compared. Unequal quantities of the two alleles were observed in 50 Serbian subjects. The low abundance allele was clearly visible only when a modified method of silver staining for polyacrylamide gels was used. This method is more reliable for Ins/Del polymorphism genotyping, avoiding misclassifications by conventional ethidium bromide staining of both agarose and polyacrylamide gels.
比较了三种检测编码信号肽的载脂蛋白B基因插入/缺失（Ins/Del）基因型的不同方法。在50名塞尔维亚受试者中观察到两个等位基因数量不等。只有当使用改良的聚丙烯酰胺凝胶银染方法时，低丰度等位基因才清晰可见。这种方法对于Ins/Del多态性基因分型更可靠，避免了琼脂糖凝胶和聚丙烯酰胺凝胶常规溴化乙锭染色导致的错误分类。
