来自产碱普罗威登斯菌O6脂多糖的含可立糖O-多糖的结构
Structure of a colitose-containing O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O6.
作者信息
Ovchinnikova Olga G, Kocharova Nina A, Wykrota Marianna, Shashkov Alexander S, Knirel Yuriy A, Rozalski Antoni
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
出版信息
Carbohydr Res. 2007 Oct 15;342(14):2144-8. doi: 10.1016/j.carres.2007.05.010. Epub 2007 May 18.
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O6 and studied by sugar and methylation analysis, selective hydrolytic removal of 3,6-dideoxy-L-xylo-hexose (colitose, Col), (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY and H-detected (1)H,(13)C HSQC and HMBC experiments. The polysaccharide was found to have a branched pentasaccharide repeating unit with the following structure: [see text] Remarkably, the trisaccharide side chain of the O6-polysaccharide represents a colitose ('3-deoxy-L-fucose') analogue of the H type 1 (precursor) antigenic determinant.
通过对产碱普罗威登斯菌O6脂多糖进行温和酸降解分离出O-多糖，并通过糖和甲基化分析、选择性水解去除3,6-二脱氧-L-木糖己糖（结肠糖，Col）、（1）H和（13）C NMR光谱（包括二维（1）H，（1）H COSY、TOCSY、ROESY以及H检测的（1）H，（13）C HSQC和HMBC实验）对其进行研究。发现该多糖具有如下结构的分支五糖重复单元：[见原文]值得注意的是，O6-多糖的三糖侧链代表H1型（前体）抗原决定簇的结肠糖（“3-脱氧-L-岩藻糖”）类似物。
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