用于蛋白质的电位酶通道免疫传感器。

Potentiometric enzyme channeling immunosensor for proteins.

作者信息

Brown D V, Meyerhoff M E

机构信息

Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.

出版信息

Biosens Bioelectron. 1991;6(7):615-22. doi: 10.1016/0956-5663(91)80027-u.

DOI:10.1016/0956-5663(91)80027-u

PMID:1756003

Abstract

A potentiometric immunosensor for the detection of human IgG has been developed using an asymmetric, ion-selective membrane with immobilized adenosine deaminase and IgG. A protein A-alkaline phosphatase conjugate binds to the immobilized IgG, creating a bienzymatic catalytic layer. In the presence of sample IgG, the conjugate does not bind to the membrane. Instead, the intermediate in the two-step reaction (adenosine) must diffuse to the membrane surface, reducing the rate of product (ammonium) formation within the diffusion layer detected by the membrane. The immunosensor demonstrated is for the determination of IgG. A simplified model is described to predict the maximum rate enhancement for the 'channeled' versus 'unchanneled' reaction mechanisms.

摘要

一种用于检测人免疫球蛋白G（IgG）的电位免疫传感器已被开发出来，它使用了一种不对称的离子选择性膜，该膜固定有腺苷脱氨酶和IgG。蛋白A-碱性磷酸酶偶联物与固定的IgG结合，形成一个双酶催化层。在存在样品IgG的情况下，偶联物不会与膜结合。相反，两步反应中的中间体（腺苷）必须扩散到膜表面，从而降低膜检测到的扩散层内产物（铵）的形成速率。所展示的免疫传感器用于测定IgG。描述了一个简化模型，以预测“通道化”与“非通道化”反应机制的最大速率增强情况。