巴西成年慢性胃炎和胃腺癌患者的白细胞介素-6基因多态性与幽门螺杆菌感染
Interleukin-6 polymorphisms, Helicobacter pylori infection in adult Brazilian patients with chronic gastritis and gastric adenocarcinoma.
作者信息
Gatti Luciano Lobo, Burbano Rommel Rodríguez, Zambaldi-Tunes Maurício, de-Lábio Roger Willian, de Assumpção Paulo Pimentel, de Arruda Cardoso-Smith Marília, Marques-Payão Spencer Luiz
机构信息
Disciplina de Genética, Hemocentro, Faculdade de Medicina de Marília, FAMEMA, Marília, São Paulo, Brazil.
出版信息
Arch Med Res. 2007 Jul;38(5):551-5. doi: 10.1016/j.arcmed.2006.12.011.
BACKGROUND
The discovery of Helicobacter pylori offered the etiologic agent of the initiating event of the inflammatory cascade. It has been confirmed that the development of gastric cancer spans over several decades sequentially starting with the acquisition of H. pylori infection and the development of chronic gastritis. The IL-6 gene (Il-6), inflammatory cytokine and the single-nucleotide polymorphisms (SNPs) at the 5' flanking region of the Il-6 gene promoter (G or C at -174 base and at -572 or -597 C or A) have been identified with increased Il-6 levels.
METHODS
Biopsies were collected from 168 patients. SNPs in Il-6-174 were analyzed by PCR-RFLP.
RESULTS
Promoter SNP of Il-6 at -174 base were within Hardy-Weinberg equilibrium. We did not find any association between the frequencies of -174 polymorphism with specific histological type of gastric adenocarcinoma, but the G (guanine) allele at -174 was significantly higher in gastric adenocarcinoma than in patients with chronic gastritis.
CONCLUSIONS
We observed an association between GG allele on -174 base with gastric cancer.
背景
幽门螺杆菌的发现为炎症级联反应起始事件提供了病原体。已证实胃癌的发展从感染幽门螺杆菌并发展为慢性胃炎开始，依次跨越数十年。白细胞介素-6基因（Il-6）、炎性细胞因子以及Il-6基因启动子5'侧翼区域的单核苷酸多态性（SNP，即-174碱基处的G或C以及-572或-597处的C或A）已被确定与Il-6水平升高有关。
方法
收集了168例患者的活检样本。通过聚合酶链反应-限制性片段长度多态性分析Il-6 -174中的SNP。
结果
Il-6在-174碱基处的启动子SNP处于哈迪-温伯格平衡。我们未发现-174多态性频率与胃腺癌特定组织学类型之间存在任何关联，但胃腺癌中-174处的G（鸟嘌呤）等位基因显著高于慢性胃炎患者。
结论
我们观察到-174碱基处的GG等位基因与胃癌之间存在关联。
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