Kim Nayoung, Cho Sung-Il, Yim Jeong-Yoon, Kim Jung Mogg, Lee Dong Ho, Park Ji Hyun, Kim Joo Sung, Jung Hyun Chae, Song In Sung
Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi-do, Korea.
Helicobacter. 2006 Apr;11(2):105-12. doi: 10.1111/j.1523-5378.2006.00384.x.
To evaluate whether or not genetic polymorphism of IL-1B, IL-1RN, and TNF-Alpha is an important factor in the different expression of gastroduodenal diseases after Helicobacter pylori infection.
This study consisted of 1360 subjects: control, gastric cancer (GC, intestinal type, and diffuse type), benign gastric ulcer (BGU), duodenal ulcer, and first-degree gastric cancer relative (GCR). IL-1Beta-511 and TNF-A-308 biallelic polymorphism were genotyped by 5' nuclease polymerase chain reaction (PCR) assays, and PCR-restriction fragment length polymorphism (PCR-RFLP). IL-1RN penta-allelic variable number of tandem repeats was genotyped by PCR.
There was no difference in the genetic polymorphism of IL-1Beta-511, IL-1RN and TNF-A in the patients with gastric cancer regardless of H. pylori positivity compared with control. However, the frequencies of IL-1B-511 C/T (OR: 0.5, 95% CI: 0.3-0.7) and T carrier (OR: 0.6, 95% CI: 0.4-0.8) were lower in the H. pylori-positive BGU patients. The IL-1RN 2/2 was higher (OR: 5.5, 95% CI: 1.1-28.5) in the H. pylori-positive GCR. There was no significance in the polymorphism of TNF-Alpha-308 regardless of H. pylori-induced gastroduodenal diseases.
The IL-1Beta-511 T-carrier polymorphism has a negative effect on the development of H. pylori-positive BGU, and high frequency of IL-1RN 2/2 was found in the H. pylori-positive relatives of GC patients, which suggest that this genetic polymorphism could play some role in the H. pylori-induced gastroduodenal diseases in Korea.
评估白细胞介素-1β（IL-1B）、白细胞介素-1受体拮抗剂（IL-1RN）和肿瘤坏死因子-α（TNF-Alpha）的基因多态性是否是幽门螺杆菌感染后胃十二指肠疾病不同表现的重要因素。
本研究包括1360名受试者：对照组、胃癌（GC，肠型和弥漫型）、良性胃溃疡（BGU）、十二指肠溃疡和一级胃癌亲属（GCR）。通过5'核酸酶聚合酶链反应（PCR）分析和PCR-限制性片段长度多态性（PCR-RFLP）对IL-1β-511和TNF-A-308双等位基因多态性进行基因分型。通过PCR对IL-1RN五等位基因串联重复可变数目进行基因分型。
与对照组相比，无论幽门螺杆菌阳性与否，胃癌患者中IL-1β-511、IL-1RN和TNF-A的基因多态性均无差异。然而，幽门螺杆菌阳性的BGU患者中IL-1B-511 C/T（比值比：0.5，95%可信区间：0.3-0.7）和T携带者（比值比：0.6，95%可信区间：0.4-0.8）的频率较低。幽门螺杆菌阳性的GCR中IL-1RN 2/2较高（比值比：5.5，95%可信区间：1.1-28.5）。无论幽门螺杆菌引起的胃十二指肠疾病如何，TNF-α-308的多态性均无显著性差异。
IL-1β-511 T携带者多态性对幽门螺杆菌阳性BGU的发生有负面影响，在GC患者的幽门螺杆菌阳性亲属中发现IL-1RN 2/2的高频率，这表明这种基因多态性可能在韩国幽门螺杆菌引起的胃十二指肠疾病中起一定作用。
