Ma Guolong, Chang Hong, Li Shiping, Chen Hongyu, Ji Dejun, Geng Rongqing, Chang Chunfang, Li Yonghong
Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China.
J Genet Genomics. 2007 May;34(5):413-9. doi: 10.1016/S1673-8527(07)60045-9.
Thirty-three mutations and four different haplotypes were found when cytochrome b(Cytb) gene partial sequences of 12 gayals were analyzed. Together with sequences of Bos indicus, Bos taurus, Bos grunniens and Bos gaurus with Bubalus bubalis as the out group, the partial sequences of Cytb gene of gayals were aligned and base composition and nucleotide variation of Cytb gene were analyzed. The phylogenetic trees were constructed by the NJ method and the MP method respectively, both supporting almost the same topology. Gayal is an independent species of Bos from Bos indicus, Bos taurus and Bos gaurus. The results also indicate that a great proportion of gayal bloodline was invaded by other species, and the protection of gayal is facing a formidable situation.
对12头大额牛的细胞色素b（Cytb）基因部分序列进行分析，发现了33个突变和4种不同单倍型。以水牛为外群，将大额牛Cytb基因部分序列与印度牛、普通牛、牦牛和白肢野牛的序列进行比对，分析了Cytb基因的碱基组成和核苷酸变异。分别采用邻接法（NJ法）和最大简约法（MP法）构建系统发育树，两者支持几乎相同的拓扑结构。大额牛是牛属中独立于印度牛、普通牛和白肢野牛的一个物种。结果还表明，大额牛的血统有很大比例受到其他物种的侵入，大额牛的保护面临严峻形势。
