Chen Yu, Chen Changjun, Wang Jianxin, Jin Lihua, Zhou Mingguo
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
J Genet Genomics. 2007 May;34(5):469-76. doi: 10.1016/S1673-8527(07)60051-4.
Twenty-two nitrate nonutilizing (nit) mutants were recovered from five wild-type isolates of Fusarium graminearum and fifty nit mutants were recovered from three JS399-19-resistant mutants of F. graminearum cultured on MMC medium. Some biological properties were compared between nit mutants and their parental isolates. The results showed that there were no significant differences in growth rate, cultural characters or pathogenicity between JS399-19-resistant nit mutants and their parental isolates. But the conidial production and the sexual reproduction ability changed to some extent. There was no cross resistance toward chlorate and JS399-19 in F. graminearum and the resistance could be stable through 20-time subcultures. Therefore, the nit could be used as a genetic marker for studying the genetics of JS399-19 resistance in F. graminearum which was used to study JS399-19 resistance transferability in hyphal fusion. Resistance in JS399-19 could not be transferred by hyphal fusion or could be transferred with low chance between two compatible isolates, which would delay the development of JS399-19 resistance in the field.
从禾谷镰刀菌的5个野生型分离株中获得了22个硝酸盐不利用（nit）突变体，从在MMC培养基上培养的3个禾谷镰刀菌JS399 - 19抗性突变体中获得了50个nit突变体。对nit突变体及其亲本分离株的一些生物学特性进行了比较。结果表明，JS399 - 19抗性nit突变体与其亲本分离株在生长速率、培养特性或致病性方面没有显著差异。但分生孢子产量和有性生殖能力在一定程度上发生了变化。禾谷镰刀菌对氯酸盐和JS399 - 19没有交叉抗性，且该抗性可通过20次传代保持稳定。因此，nit可作为研究禾谷镰刀菌中JS399 - 19抗性遗传学的遗传标记，用于研究菌丝融合中JS399 - 19抗性的转移性。JS399 - 19的抗性不能通过菌丝融合转移，或者在两个亲和分离株之间以低概率转移，这将延缓田间JS399 - 19抗性的发展。
