Yeung Leo W Y, Guruge Keerthi S, Yamanaka Noriko, Miyazaki Shigeru, Lam Paul K S
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-0856, Japan; Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Safety Research Team, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki 305-0856, Japan.
Toxicology. 2007 Jul 31;237(1-3):111-125. doi: 10.1016/j.tox.2007.05.004. Epub 2007 May 6.
The effects of PFOS and PFOA on the gene expression patterns of chickens that were exposed to either PFOS or PFOA at low doses were investigated with the use of microarray techniques. Twelve Genechip Chicken Genome Arrays were used to study hepatic gene expression in 6-week-old chickens (Gallus gallus) that were exposed to either PFOA (0.1, 0.5, or 5mg/mL), PFOS (0.02 or 0.1mg/mL), or a saline vehicle control (0.9% NaCl in Milli-Q water) via subcutaneous implantation of a 2mL osmotic pump for 4 weeks or for 4 weeks with a further 4 weeks of depuration. Over 240 and 480 genes were significantly affected by PFOS after 4 weeks of exposure and after 4 weeks of exposure with a further 4 weeks of depuration, respectively and over 290 and 320 genes were significantly affected by PFOA, correspondingly. For PFOS, the genes that were affected after 4 weeks of exposure were mainly related to the transport of electrons and oxygen, and the metabolism of lipids and fatty acids; while the genes that were affected after 4 weeks of exposure with a further 4 weeks of depuration were mainly related to the transport of electrons and ions, and protein amino acid phosphorylation and proteolysis. For PFOA, the genes that were affected after 4 weeks of exposure were related to the transport of ions, lipids, and electrons and cytochromes; while the genes that were affected after 4 weeks of exposure with a further 4 weeks of depuration were related to protein amino acid phosphorylation and proteolysis, the transport of ions, and the metabolism of fatty acids and lipids. The results also showed that the gene expression patterns between chickens that were treated with PFOS and those that were treated with PFOA were different, which points to the importance of the separate evaluation of the toxicities of PFOS and PFOA. Specifically, the gene expressions of CYP8B and NOV were studied.
利用微阵列技术研究了低剂量全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）对暴露于其中的鸡基因表达模式的影响。使用12张基因芯片鸡基因组阵列研究6周龄鸡（原鸡）的肝脏基因表达，这些鸡通过皮下植入2mL渗透泵暴露于PFOA（0.1、0.5或5mg/mL）、PFOS（0.02或0.1mg/mL）或生理盐水载体对照（超纯水配制的0.9%NaCl）中4周，或暴露4周后再净化4周。暴露4周后和暴露4周再净化4周后，分别有超过240个和480个基因受到PFOS的显著影响，相应地，有超过290个和320个基因受到PFOA的显著影响。对于PFOS，暴露4周后受影响的基因主要与电子和氧气运输、脂质和脂肪酸代谢有关；而暴露4周再净化4周后受影响的基因主要与电子和离子运输、蛋白质氨基酸磷酸化和蛋白水解有关。对于PFOA，暴露4周后受影响的基因与离子、脂质和电子运输以及细胞色素有关；而暴露4周再净化4周后受影响的基因与蛋白质氨基酸磷酸化和蛋白水解、离子运输以及脂肪酸和脂质代谢有关。结果还表明，PFOS处理组和PFOA处理组鸡的基因表达模式不同，这表明分别评估PFOS和PFOA毒性的重要性。具体而言，研究了CYP8B和NOV的基因表达。
