Neurogenesis of GABAergic cells in the retina of malnourished rats.

The present study investigated how prenatal protein malnutrition affects the neurogenesis of GABAergic cells in the retina. Rats were treated with a multi-deficient diet, with only 8% of protein that was administered during the gestational and suckling periods. Pregnant mothers and pups from malnourished and control (fed with 22% protein) groups received a single intra-peritoneal injection of [3H]-thymidine at six developmental ages, from E14 to PN4, and the pups were sacrificed at PN18. Eyes were enucleated and cryosections of the retina were double labeled for GABA-immunocytochemistry and for autoradiography. The percentage of double labeled cells, in the retinal inner nuclear and ganglion cell layers, was determined for both groups. Qualitative and quantitative results showed that double labeled cells [GABA+/thymidine+] were present since E14, when mitotic activity for GABAergic cells starts, in both GCL and INL layers. The peak rate of GABAergic cell generation was reached in control animals injected with [3H]-thymidine at E18 in both central and peripheral sectors of the retina, but only at E20 in the malnourished group. The generation of cells of GABA phenotype showed a significant delay in both layers of the retina in the malnourished group. At PN4, close to the age that GABAergic mitotic activity ends in the control group, double labeled cells were significantly higher in the malnourished group. Our data showed a delay in GABAergic cell generation in the malnourished group when compared to the control group that might result in significant functional consequences in the developing retina.