Torimoto Y, Nagoya S, Uede T
Department of Pathology, Sapporo Medical College, Japan.
Int Immunol. 1991 Oct;3(10):949-57. doi: 10.1093/intimm/3.10.949.
A signal transduction was detected in various rat T cells by cross-linking of 8H3 antigen by 8H3 antibody. A rat T cell proliferative response induced by 8H3 antibody is dependent on the presence of adherent cells. When spleen cells were cultured in the presence of 8H3 antibody, only CD4-positive T cell proliferation was induced. These proliferative CD4-positive T cells express rat interleukin 2 receptor, alpha chain. Cross-linking of 8H3 antigen resulted in an increase in cytoplasmic free Ca2+ in T cells and phosphorylation of a 120 kDa component of the 8H3 antigen. It should be noted that cross-linking of 8H3 antigen together with CD4 antigen but not with CD8 antigen by suboptimal doses of 8H3 and corresponding antibodies initiated the mobilization of [Ca2+]i. Furthermore, physical association of 8H3 antigen with TCR was demonstrated by comodulation of these two molecular complexes in some T cells. Thus, 8H3 antigen is involved in rat T cell transmembrane signal transduction.
通过8H3抗体交联8H3抗原，在多种大鼠T细胞中检测到信号转导。8H3抗体诱导的大鼠T细胞增殖反应依赖于黏附细胞的存在。当脾细胞在8H3抗体存在下培养时，仅诱导CD4阳性T细胞增殖。这些增殖的CD4阳性T细胞表达大鼠白细胞介素2受体α链。8H3抗原的交联导致T细胞中细胞质游离Ca2+增加以及8H3抗原120 kDa成分的磷酸化。应当注意的是，次优剂量的8H3和相应抗体与CD4抗原而非CD8抗原一起交联8H3抗原引发了[Ca2+]i的动员。此外，在一些T细胞中，通过这两种分子复合物的共调节证明了8H3抗原与TCR的物理关联。因此，8H3抗原参与大鼠T细胞跨膜信号转导。
