[肿瘤转移抑制基因KiSS-1和基质金属蛋白酶-9在肝细胞癌门静脉癌栓中的表达]

[Expression of tumor metastasis-suppressor gene KiSS-1 and matrix metalloproteinase-9 in portal vein tumor thrombus of hepatocellular carcinoma].

作者信息

Hou Yuan-Kai, Wang Yi, Cong Wen-Ming, Wu Meng-Chao

机构信息

Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P. R. China.

出版信息

Ai Zheng. 2007 Jun;26(6):591-5.

PMID:17562263

Abstract

BACKGROUND & OBJECTIVE: Tumor metastasis-suppressor gene KiSS-1 is related to the metastasis of malignancies. However, its correlation to the metastasis, especially the formation of portal vein tumor thrombus (PVTT), of hepatocellular carcinoma (HCC) has seldom been reported. This study was to investigate the function of KiSS-1 gene in the formation of PVTT of HCC, and explore the machanism.

METHODS

The expression of KiSS-1 and matrix metalloproteinase-9 (MMP-9) in 50 specimens of HCC (31 cases with PVTT and 19 cases without PVTT) and 11 specimens of PVTT was detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC).

RESULTS

The positive rates of KiSS-1 mRNA and protein were significantly lower in PVTT group and HCC with PVTT group than in HCC without PVTT group (18.2% and 16.1 % vs. 63.2%, 0% and 12.9% vs. 47.4%, P < 0.05)û the positive rates of MMP-9 mRNA and protein were significantly higher in PVTT group and HCC with PVTT group than in HCC without PVTT group (72.7% and 77.4% vs. 31.6%, 81.8% and 83.9% vs. 42.1%, P < 0.05). There was no significant difference between PVTT group and HCC with PVTT group (P > 0.05). KiSS-1 expression was negatively related to MMP-9 expression (r=-0.362 for mRNA, and r=-0.473 for protein, each P < 0.05).

CONCLUSIONS

KiSS-1 and MMP-9 are closely related to the formation of PVTT. KiSS-1 gene might suppress the formation of PVTT by suppressing MMP-9 synthesis.

摘要

背景与目的

肿瘤转移抑制基因KiSS-1与恶性肿瘤转移相关。然而，其与肝细胞癌（HCC）转移尤其是门静脉癌栓（PVTT）形成的相关性鲜有报道。本研究旨在探讨KiSS-1基因在HCC的PVTT形成中的作用并探索其机制。

方法

采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学（IHC）检测50例HCC标本（31例有PVTT，19例无PVTT）及11例PVTT标本中KiSS-1和基质金属蛋白酶-9（MMP-9）的表达。

结果

PVTT组及有PVTT的HCC组中KiSS-1 mRNA和蛋白的阳性率显著低于无PVTT的HCC组（18.2%和16.1% 对 63.2%，0%和12.9% 对 47.4%，P<0.05）；PVTT组及有PVTT的HCC组中MMP-9 mRNA和蛋白的阳性率显著高于无PVTT的HCC组（72.7%和77.4% 对31.6%，81.8%和83.9% 对42.1%，P<0.05）。PVTT组与有PVTT的HCC组之间无显著差异（P>0.05）。KiSS-1表达与MMP-9表达呈负相关（mRNA方面r=-0.362，蛋白方面r=-0.473，均P<0.05）。